NDLI: Hes1 inhibitor isolated by target protein oriented natural products isolation (TPO-NAPI) of differentiation activators of neural stem cells

Content Provider	Royal Society of Chemistry (RSC)
Author	Tanaka, Mitsuha Suganami, Akiko Kowithayakorn, Thaworn Ishibashi, Masami Ishikawa, Naoki Uemura, Kenji Sugimitsu, Noriko Koyano, Takashi Arai, Midori A. Tamura, Yutaka
Copyright Year	2016
Abstract	The Hes1 dimer inhibitor, agalloside (2), which can accelerate the differentiation of neural stem cells is described. Six natural products, including one new natural product, which bind to Hes1 were rapidly isolated by a developed “target protein oriented natural products isolation” (TPO-NAPI) method using Hes1-immobilized beads. Of the six compounds, 2 inhibited Hes1 dimer formation at both the protein- and cellular level. Neural stem cells treated with 2 differentiated to neurons with longer neurites than cells treated with varproic acid or retinoic acid. Moreover, 2 exhibited specificity for neurons. This promotion of differentiation was supported by an increase in the mRNA expression of the proneural genes, Mash1 and Ngn2, which were inhibited by Hes1.
Starting Page	1514
Ending Page	1520
Page Count	7
File Format	HTM / HTML PDF
ISSN	20416520
Volume Number	7
Issue Number	2
Journal	Chemical Science
DOI	10.1039/c5sc03540f
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Acid Messenger RNA Enzyme inhibitor Natural product Protein dimer Protein
Content Type	Text
Resource Type	Article
Subject	Chemistry

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Hes1 inhibitor isolated by target protein oriented natural products isolation (TPO-NAPI) of differentiation activators of neural stem cells