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lated by target protein oriented natural products isolation ( TPO-NAPI ) of di ff erentiation activators of neural stem cells †
| Content Provider | Semantic Scholar |
|---|---|
| Author | Arai, Midori A. Ishikawa, Naoki Tanaka, Mitsuha Uemura, Kenji Sugimitsu, Noriko Suganami, Akiko Tamura, Yutaka Koyano, Takashi Kowithayakornd, Thaworn Ishibashi, Masami |
| Copyright Year | 2016 |
| Abstract | The Hes1 dimer inhibitor, agalloside (2), which can accelerate the differentiation of neural stem cells is described. Six natural products, including one new natural product, which bind to Hes1 were rapidly isolated by a developed “target protein oriented natural products isolation” (TPO-NAPI) method using Hes1-immobilized beads. Of the six compounds, 2 inhibited Hes1 dimer formation at both the proteinand cellular level. Neural stem cells treated with 2 differentiated to neurons with longer neurites than cells treated with varproic acid or retinoic acid. Moreover, 2 exhibited specificity for neurons. This promotion of differentiation was supported by an increase in the mRNA expression of the proneural genes, Mash1 and Ngn2, which were inhibited by Hes1. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://pubs.rsc.org/en/content/articlepdf/2016/sc/c5sc03540f |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
