NDLI: Sequence-responsive unzipping DNA cubes with tunable cellular uptake profiles

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Sequence-responsive unzipping DNA cubes with tunable cellular uptake profiles

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Back cover

Chemical Science Vol - 5 Issue - 5

Chemical Science Vol - 5 Issue - 4

Chemical Science Vol - 5 Issue - 3

Chemical Science Vol - 5 Issue - 2

Chemical Science Vol -4

Chemical Science Vol -3

Chemical Science Vol -2

Chemical Science Vol -1

Sequence-responsive unzipping DNA cubes with tunable cellular uptake profiles

Content Provider	Royal Society of Chemistry (RSC)
Author	Edwardson, Thomas G. W. Godin, Antoine G. Amrein, Lilian Wiseman, Paul W. Bujold, Katherine E. Fakhoury, Johans Hamblin, Graham D. Briard, Joel Neves Sleiman, Hanadi F. Panasci, Lawrence C. Carneiro, Karina M. M.
Copyright Year	2014
Abstract	Here, we demonstrate a new approach for the design and assembly of a dynamic DNA cube with an addressable cellular uptake profile. This cube can be selectively unzipped from a 3D to a flat two-dimensional structure in the presence of a specific nucleic acid sequence. Selective opening is demonstrated in vitro using a synthetic RNA marker unique to the LNCaP human prostate cancer cell line. A robust uptake in LNCaP cells, HeLa cells (human cervical cancer) and primary B-lymphocytes isolated from the blood of chronic lymphocytic leukemia (CLL) patients is observed using fluorescence-activated cell sorting (FACS), confocal microscopy and a new cluster analysis algorithm combined with image cross-correlation spectroscopy. The DNA cube was modified with hydrophobic and hydrophilic dendritic chains that were found to coat its exterior. The dynamic unzipping properties of these modified cubes were retained, and assessment of cellular uptake shows that the hydrophobic chains help with the rapid uptake of the constructs while the hydrophilic chains become advantageous for long term internalization.
Starting Page	2449
Ending Page	2455
Page Count	7
File Format	HTM / HTML PDF
ISSN	20416520
Volume Number	5
Issue Number	6
Journal	Chemical Science
DOI	10.1039/c4sc00646a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Cluster analysis Flow cytometry Spectroscopy Acid LNCaP B-cell chronic lymphocytic leukemia Confocal microscopy Hydrophobe Algorithm Cross-correlation Three-dimensional space HeLa B cell Messenger RNA LPI Media DNA Hydrophile Prostate cancer Cervical cancer
Content Type	Text
Resource Type	Article
Subject	Chemistry

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