NDLI: Hydrogelation of dextran-based polyampholytes with cryoprotective properties <i>via</i> click chemistry

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Biomaterials Science Vol - 2 Issue - 2

Biomaterials Science Vol -1

Hydrogelation of dextran-based polyampholytes with cryoprotective properties via click chemistry

Content Provider	Royal Society of Chemistry (RSC)
Author	Rajan, Robin Jain, Minkle Hyon, Suong-Hyu Matsumura, Kazuaki
Copyright Year	2014
Abstract	Hydrogels are promising substrates for tissue engineering applications because of their unique biocompatibility, flexible methods of synthesis, range of constituents, and desirable physical characteristics. Cryopreservation of cell-containing constructs using such hydrogel scaffolds is in high demand in tissue-engineering applications for the production of “off-the-shelf” tissue-engineered products. However, cryopreservation of regenerated tissues including cell sheets and cell constructs is not easy compared to the preservation of cell suspensions, even when cryoprotectants are used. Here, we report a dextran-based polyampholyte hydrogel that itself shows cryoprotective properties, which could be useful for cell encapsulation and tissue engineering applications involving hydrogel formation. Amination was performed by introducing poly-L-lysine onto azide groups conjugated with dextran, and a portion of the amino groups was converted into carboxyl groups. These dextran-based polyampholytes showed good cryoprotective properties for mammalian cells, and the addition of dextran substituted with dibenzylcyclooctyne acid induced in situ hydrogel formation via Cu-free click chemistry with high biocompatibility. Cells encapsulated with such in situ hydrogels can be cryopreserved well without the addition of any cryoprotectants. Thus, these hydrogels can serve as scaffolds with cryoprotective properties that also provide structural integrity to tissue constructs.
Starting Page	308
Ending Page	317
Page Count	10
File Format	HTM / HTML PDF
ISSN	20474830
Volume Number	2
Issue Number	3
Journal	Biomaterials Science
DOI	10.1039/c3bm60261c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Dextran Amination Tissue engineering Cryopreservation Cryoprotectant Carboxylic acid Biocompatibility Click chemistry Azide Free Gel
Content Type	Text
Resource Type	Article
Subject	Materials Science Biomedical Engineering

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