NDLI: Mesenchymal stem cell response to TGF-β1 in both 2D and 3D environments

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Biomaterials Science Vol - 1 Issue - 7

Biomaterials Science Vol - 1 Issue - 6

Biomaterials Science Vol - 1 Issue - 5

Biomaterials Science Vol - 1 Issue - 4

Biomaterials Science Vol - 1 Issue - 3

Biomaterials Science Vol - 1 Issue - 2

Mesenchymal stem cell response to TGF-β1 in both 2D and 3D environments

Content Provider	Royal Society of Chemistry (RSC)
Author	Suggs, Laura J. Stowers, Ryan S. Chung, Eunna Drinnan, Charles T.
Copyright Year	2013
Abstract	Smooth muscle cells (SMC) are critical in stabilizing developing vascular networks, and transforming growth factor β1 (TGF-β1) has been shown to promote SMC differentiation from stem cells. Previously, our lab has developed a chemically modified fibrin-based hydrogel that induces endothelial cell (EC) phenotype and network formation from human mesenchymal stem cells (hMSCs) without exogenous cytokines. Additionally, we have shown that this hydrogel system is capable of releasing growth factors in a controlled manner. In the present work, the effects of TGF-β1 on hMSCs in both monolayer and fibrin-based gel culture systems were demonstrated. The objective was to enhance SMC properties through TGF-β1 signaling for vessel stability while maintaining EC gene expression and morphology. Proliferation was decreased with higher TGF-β1 concentration in both monolayer and 3D gel cultures. EC genes were predominantly downregulated in the presence of TGF-β1 in monolayer cultures, while SMC genes were generally upregulated. In fibrin-based gels, several SMC genes were significantly upregulated at high concentrations of TGF-β1. Even at elevated TGF-β1 concentrations, no significant differences were seen in EC genes for hMSCs in gels compared to controls. Network formation and growth occurred in PEGylated fibrin gels loaded with TGF-β1 and were not significantly different from gels without loaded growth factor. Additionally, production of smooth muscle α-actin (SMA) was significantly increased in gels loaded with TGF-β1. These results demonstrate a simultaneous response of hMSCs to both the 3D biomatrix and cytokine signaling cues.
Starting Page	860
Ending Page	869
Page Count	10
File Format	HTM / HTML PDF
ISSN	20474830
Volume Number	1
Issue Number	8
Journal	Biomaterials Science
DOI	10.1039/c3bm60057b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Mesenchymal stem cell Fibrin Phenotype Gene Cytokine Smooth muscle tissue EC Growth factor Gel Endothelium Three-dimensional space
Content Type	Text
Resource Type	Article
Subject	Materials Science Biomedical Engineering

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