NDLI: Bone marrow mesenchymal stem cells combined with calcium alginate gel modified by hTGF-β1 for the construction of tissue-engineered cartilage in three-dimensional conditions.

Please wait, while we are loading the content...

Bone marrow mesenchymal stem cells combined with calcium alginate gel modified by hTGF-β1 for the construction of tissue-engineered cartilage in three-dimensional conditions.

Content Provider	Europe PMC
Author	ZHU, SHAOBO ZHANG, TAO SUN, CHEN YU, AIXI QI, BAIWEN CHENG, HAO
Copyright Year	2013
Abstract	The aim of this study was to investigate the feasibility of Ad-hTGF-β1-transfected bone marrow mesenchymal stem cells (BMMSCs) combined with calcium alginate gel for the construction of tissue-engineered cartilage under three-dimensional conditions. Rat BMMSCs were divided into three groups: the Ad-hTGF-β1 transfection group, the Ad-EGFP transfection group and the control group. The BMMSCs in the Ad-hTGF-β1 transfection group were continually grown. The compound of cell-calcium alginate gel was cultured in a constant temperature incubator. The morphology of cells was examined, and the proliferation of cells was detected by MTT assay. The results from real-time PCR showed that the average relative ratio of TGF-β1 and transcriptional coactivator with PDZ-binding motif (TAZ) in the Ad-hTGF-β1 group was comparable to that of the control group (P<0.05). Using western blotting and immunohistochemistry, strong expression of collagen II in the Ad-hTGF-β1 group was detected. The results from western blotting showed that the expression of TGF-β1 in the Ad-hTGF-β1 group was significantly increased compared with that of the other two groups. The differentiation of BMMSCs was induced by Ad-hTGF-β1 transfection into chondrocytes. TGF-β1 may promote the differentiation of BMMSCs into chondrocytes by TAZ. BMMSCs transfected by Ad-hTGF-β1 could be induced into chondrocytes. These three-dimensional conditions could preferably mimic cell growth patterns in vivo.
ISSN	17920981
Journal	Experimental and Therapeutic Medicine
Volume Number	5
PubMed Central reference number	PMC3524118
Issue Number	1
PubMed reference number	23251248
e-ISSN	17921015
DOI	10.3892/etm.2012.765
Language	English
Publisher	D.A. Spandidos
Publisher Date	2012-10-25
Access Restriction	Open
Rights License	This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. Copyright © 2013, Spandidos Publications
Subject Keyword	gene transfection chondrocytes TGF-β1 TAZ three-dimensional culture
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Cancer Research

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in