NDLI: Stabilizing viral nano-reactors for nerve-agent degradation

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Stabilizing viral nano-reactors for nerve-agent degradation

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Biomaterials Science Vol - 1 Issue - 7

Biomaterials Science Vol - 1 Issue - 6

Biomaterials Science Vol - 1 Issue - 5

Biomaterials Science Vol - 1 Issue - 4

Biomaterials Science Vol - 1 Issue - 3

Biomaterials Science Vol - 1 Issue - 2

Stabilizing viral nano-reactors for nerve-agent degradation

Content Provider	Royal Society of Chemistry (RSC)
Author	O'Neil, Alison Douglas, Trevor Prevelige, Peter E.
Copyright Year	2013
Abstract	Virus like particles, and other naturally occurring protein containers, have emerged as excellent building blocks for nanomaterials design and synthesis. Here we exploit a directed assembly and encapsulation approach to sequester multiple copies of a phosphotriesterase (PTE) enzyme within the capsid of bacteriophage P22. Phosphotriesterase, from Brevundimonas diminuta, is an intriguing enzyme as it is highly active against a wide range of harmful insecticides and nerve agents such as Soman and Sarin. However, difficulty in expressing large quantities of the active recombinant enzyme has limited efforts to scale-up its use. Additionally, as a mesophilic enzyme its low heat tolerance and susceptibility to proteolysis makes it a less than ideal candidate as a practical bioremediation tool. Through encapsulation of the PTE within the P22 capsid, we demonstrate a greatly enhanced thermal tolerance of the enzyme, maintaining 50% of its activity to 60 °C. Additionally, the P22 capsid confers protection to the enzyme from proteases, as well as stabilizing the enzyme against desiccation. Thus, our engineered P22 encapsulation system greatly enhances the stability of the mesophilic phosphotriesterase and results in a robust and active nanoparticle reactor.
Starting Page	881
Ending Page	886
Page Count	6
File Format	HTM / HTML PDF
ISSN	20474830
Volume Number	1
Issue Number	8
Journal	Biomaterials Science
DOI	10.1039/c3bm60063g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	PTE Enzyme Nanoparticle Desiccation Nanomaterials Mesophile Bacteriophage Nerve agent Protein Brevundimonas Soman Bioremediation Capsid
Content Type	Text
Resource Type	Article
Subject	Materials Science Biomedical Engineering

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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