NDLI: Ultra-performance liquid chromatographic assay coupled with two-dimensional separation for spectrometric determination of urinary <i>S</i>-phenylmercapturic acid

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Ultra-performance liquid chromatographic assay coupled with two-dimensional separation for spectrometric determination of urinary S-phenylmercapturic acid

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Ultra-performance liquid chromatographic assay coupled with two-dimensional separation for spectrometric determination of urinary S-phenylmercapturic acid

Content Provider	Royal Society of Chemistry (RSC)
Author	Ong, Her Yam Ong, Choon Nam Lee, Bee Lan Chia, Sin Eng
Copyright Year	2011
Abstract	A gradient ultra-performance liquid chromatographic (UPLC) assay coupled with the two dimensional separation and diode array detection method was developed to determine S-phenylmercapturic acid (SPMA), a specific urinary metabolite of benzene. Gradient separation was performed on two C18 core–shell columns (2.6 and 1.7 μm, 50 × 2.1 mm i.d.) maintained at 40 and 20 °C. Among various solvents evaluated, dichloromethane which offered >85% recovery rates of both SPMA and D-benzylmercapturic acid (internal standard) was used for extraction. The yield of free-SPMA was maximized by hydrolyzing 200 μL of urine with 20 μL of 6 M HCl prior to dichloromethane extraction. The limit of detection was 1 μg L−1(s/n = 5) and the limit of quantification was 2 μg L−1. Using our method, a correlation of 0.99 was obtained with 10 specimens (range, 1.7 to 182 μg L−1) from the German External Quality Assessment Scheme. Among the 75 non-occupational exposure individuals, 61 (81%) had SPMA levels below the LOQ. Among the 26 male petroleum workers, 5 who were exposed to <0.04 ppm of benzene were found to have SPMA levels below the LOQ. The geometric means obtained from the other 15 non-smokers and 6 smokers who were exposed to <0.5 ppm of benzene were 5.05 and 6.65 μg g−1creatinine, respectively. The proposed method can be useful for occupational benzene exposure surveillance.
Starting Page	2025
Ending Page	2031
Page Count	7
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	3
Issue Number	9
Journal	Analytical Methods
DOI	10.1039/c1ay05089c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Urine Hydrochloric acid Acid Metabolite Benzene Micrometre Dichloromethane Chromatography Light-emitting diode
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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