NDLI: On-chip real-time nucleic acid sequence-based amplification for RNA detection and amplification

Content Provider	Royal Society of Chemistry (RSC)
Author	Bahi, Mahadji M. Tsaloglou, Maria-Nefeli Mowlem, Matthew Morgan, Hywel Waugh, Edward M.
Copyright Year	2011
Abstract	Development of integrated systems for nucleic acid analyses is mainly driven by the requirement for fast and simple clinical and environmental diagnostics. The need for affordable and effective point-of-care diagnosis has inspired an entire field of biotechnology in micro and nano-fluidics. We are developing a microfluidic system that has individual sub-systems for performing cell concentration and lysis, RNA extraction/purification and real-time RNA detection. The system is being developed to analyse the rbcL gene of phytoplankton Karenia brevis, a species responsible for harmful algal blooms. This integrated system will perform sub-cellular analysis of RNA using nucleic acid sequence-based amplification and would be used in large scale biochemical analysis and experimentation. The device could potentially be used for the detection of any species with a known target nucleic acid sequence for in situ environmental monitoring, forensics or clinical diagnostics.
Starting Page	2127
Ending Page	2133
Page Count	7
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	3
Issue Number	9
Journal	Analytical Methods
DOI	10.1039/c1ay05164d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Biotechnology RNA extraction Algal bloom Acid RNA Lysis RuBisCO DNA sequencing
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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