NDLI: Tetrazolium salt based methods for high-throughput evaluation of anti-parasite chemotherapy

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Tetrazolium salt based methods for high-throughput evaluation of anti-parasite chemotherapy

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Analytical Methods Vol - 3 Issue - 8

Analytical Methods Vol - 3 Issue - 7

Analytical Methods Vol - 3 Issue - 6

Analytical Methods Vol - 3 Issue - 5

Analytical Methods Vol - 3 Issue - 4

Analytical Methods Vol - 3 Issue - 3

Analytical Methods Vol - 3 Issue - 2

Analytical Methods Vol -2

Analytical Methods Vol -1

Tetrazolium salt based methods for high-throughput evaluation of anti-parasite chemotherapy

Content Provider	Royal Society of Chemistry (RSC)
Author	Henriques, Cristina Souza, Wanderley de Maia-Brigagão, Claudia Carvalho, Técia Maria U. Moreira, Thiago Luiz B. Henriques-Pons, Andréa
Copyright Year	2011
Abstract	This study demonstrates the first standardization of the MTS/PMS tetrazolium-based method in T. cruzi and G. duodenalis, and its application for chemotherapy studies and drug screening. We improved the detection limit and compared the MTT, XTT/PMS and MTS/PMS methods, in T. cruzi epimastigotes, demonstrating that MTS/PMS is more reproducible, sensitive, faster and easier to manipulate than XTT/PMS and MTT. Oligomycin (10 μg ml−1) reduced T. cruzi epimastigote metabolic activity by 60% measured by the MTS/PMS method, which is in accordance with flow cytometry measurements. To validate the MTS/PMS method, an IC50 of 10.5 μM was estimated for the drug amiodarone in T. cruzi and an IC50 of 6.2 μM for metronidazole in G. duodenalis, corroborating the IC50 values found in the literature. In G. duodenalis, a protozoan that has remnant mitochondria, the MTS/PMS method displayed higher sensitivity than T. cruzi epimastigotes. The detection limit was 104 for G. duodenalis trophozoites and 2.5 × 105 for T. cruzi epimastigotes. The intensity of the colored MTS/PMS formazan derivative is proportional to the number of metabolically active protozoa, regardless of whether used in a mitochondrial or an “amitochondrial” organism.
Starting Page	2148
Ending Page	2155
Page Count	8
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	3
Issue Number	9
Journal	Analytical Methods
DOI	10.1039/c1ay05219e
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Oligomycin Mitochondrion Flow cytometry Protozoa Chemotherapy Formazan IC50 Amiodarone Metronidazole MTT Trypanosomatida
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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A water-soluble tetrazolium salt useful for colorimetric cell viability assay

Coaxial Flow System for Chemical Cytometry

Notes Benzothiazole-Containing Tetrazolium Salts That Produce Water-Soluble Formazan Dyes Absorbing at a Long Wavelength upon NADH Reduction

Interfering with Color Response by Porphyrin-Related Compounds in the MTT Tetrazolium-Based Colorimetric Assay.

A New Sulfonated Tetrazolium Salt That Produces a Highly Water-Soluble Formazan Dye

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High throughput flow cytometry

Integrated pneumatic micro-pumps for high-throughput droplet-based microfluidics

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Tetrazolium salt based methods for high-throughput evaluation of anti-parasite chemotherapy

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