NDLI: Chitosan/carbon nanotube composites for the isolation of hemoglobin in the presence of abundant proteins

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Chitosan/carbon nanotube composites for the isolation of hemoglobin in the presence of abundant proteins

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Chitosan/carbon nanotube composites for the isolation of hemoglobin in the presence of abundant proteins

Content Provider	Royal Society of Chemistry (RSC)
Author	Chen, Xuwei Wang, Jianhua Wang, Wenjing Song, Zhining
Copyright Year	2011
Abstract	A cross-linked chitosan/multi-wall carbon nanotube (MWCNTs) nanocomposite is prepared via surface deposition-cross linking. The nanocomposite is characterized by means of FT-IR, SEM and TGA analysis, indicating that a chitosan layer is well coated on the surfaces of the MWCNTs, weighing about 35% of the total mass. Investigations on the performance of the chitosan/MWCNTs nanocomposite to the adsorption or loading of hemoglobin indicated that the adsorption equilibrium time is reduced from a few hours in the conventional protocols to 25 min in the present case. A theoretical adsorption capacity of 15.41 μg mg−1 is achieved, corresponding to a 6-fold improvement when compared to that obtained by using conventional chitosan particles in the micrometre range at a same mass condition. The nanocomposite is used as an adsorbent for the isolation of hemoglobin from human whole blood, the SDS-PAGE results indicated that successful separation of hemoglobin is obtained in the presence of large amount of abundant protein, e.g., human serum albumin.
Starting Page	1769
Ending Page	1773
Page Count	5
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	3
Issue Number	8
Journal	Analytical Methods
DOI	10.1039/c1ay05130j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Carbon nanotube Polyacrylamide gel electrophoresis Human serum albumin Adsorption Micrometre Hemoglobin Nanocomposite Chitosan Fourier-transform infrared spectroscopy Protein
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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Chitosan/carbon nanotube composites for the isolation of hemoglobin in the presence of abundant proteins

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