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| Content Provider | Royal Society of Chemistry (RSC) |
|---|---|
| Author | Wang, Jian-Hua Chen, Shuai Chen, Xu-Wei Yang, Ting Liu, Jia-Wei |
| Copyright Year | 2013 |
| Abstract | A novel functionalized graphene-based composite is prepared by successive modification of graphene oxide (GO) with epichlorohydrin (ECH), iminodiacetic acid (IDA) and 1-phenylboronic acid (1-PBA) through covalent functionalization and then chelating with nickel ions. Characterizations by FT-IR, XRD, SEM, TGA and ICP-MS demonstrate the successful modification of the graphene surface, resulting in a GO–PBA–IDA–Ni composite with a Ni2+ immobilization amount of 3.01 × 10−3 mol g−1. The composite shows favorable selectivity for the adsorption of lysozyme (Lys). In practice, a homogeneous GO–PBA–IDA–Ni film with a thickness of ca. 1.0 μm is fabricated by filtering the composite through a cellulose membrane with a pore aperture of 1.2 μm. The GO–PBA–IDA–Ni film is subsequently fastened into a rounded membrane cartridge and incorporated into a sequential injection system for on-line selective isolation of Lys. An adsorption efficiency of ca. 96% is achieved for 25 μg ml−1 Lys in 500 μl of sample solution at a loading rate of 5 μl s−1. Metal affinity and electrostatic interactions are the main driving forces for governing the protein adsorption behaviors. The retained Lys is readily recovered by a borate buffer (pH 10) containing 1.0 mol l−1 NaCl and 20 mmol l−1 imidazole, giving rise to a recovery of ca. 90%. The practical applicability of the composites is demonstrated by selective isolation of Lys from chicken egg white, and SDS-PAGE assay indicates that Lys is selectively isolated with high purity from complex matrices. |
| Starting Page | 810 |
| Ending Page | 818 |
| Page Count | 9 |
| File Format | HTM / HTML PDF |
| ISSN | 2050750X |
| Volume Number | 1 |
| Issue Number | 6 |
| Journal | Journal of Materials Chemistry B |
| DOI | 10.1039/c2tb00334a |
| Language | English |
| Publisher | Royal Society of Chemistry |
| Access Restriction | Open |
| Subject Keyword | SEM PBA Lys NaCl Graphene Epichlorohydrin Acid Philippine Basketball Association Covalent bond Nickel Fourier-transform infrared spectroscopy X-ray crystallography Adsorption Lysozyme Lysine Micrometre Cellulose Aperture Protein Borate PH Imidazole Chicken Egg white Polyacrylamide gel electrophoresis |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Medicine Materials Science Biomedical Engineering |
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