NDLI: Surface Assembly of Graphene Oxide Nanosheets on $SiO_{2}$ Particles for the Selective Isolation of Hemoglobin

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Surface Assembly of Graphene Oxide Nanosheets on $SiO_{2}$ Particles for the Selective Isolation of Hemoglobin

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wang, Jian-hua Zhang, Qian Chen, Xu-wei Liu, Jia-wei
Description	Author Affiliation: Liu JW ( Research Center for Analytical Sciences, Box 332, Northeastern University, Shenyang 110819, PR China.)
Abstract	Graphene oxide (GO) nanosheets have been immobilized onto $SiO_{2}$ particles through electrostatic interactions by surface assembly. The surface-assembled composite material was characterized by means of SEM and FTIR and UV/Vis spectroscopy to reveal an assembling ratio of 2.3 % (w/w, $GO/SiO_{2}).$ The $GO/SiO_{2}$ composites were subsequently used, for the first time, as adsorbents for the adsorption/isolation of proteins. Selective isolation of proteins of interest, namely, hemoglobin (Hb) in this case, from complex sample matrices, for example, human whole blood, could be obtained by carefully manipulating the adsorption/desorption process. At pH 7, an adsorption of 85 % was achieved for Hb $(70 mg L^{−1})$ in sample solution (1.0 mL) by the $GO/SiO_{2}$ composites (3.0 mg). The adsorption behavior was consistent with the Langmuir adsorption model, corresponding to a theoretical adsorption capacity of $50.5 mg g^{−1}$ for Hb. The retained Hb could be readily recovered by using a Tris-HCl buffer at pH 8.9 to give a recovery of 80 %. Circular dichroism and specific activity investigations indicated that the $GO/SiO_{2}$ composites exhibited favorable biocompatibility, characterized by virtually no effect on the conformation and activity of Hb after adsorption/desorption. The composites were used for the selective isolation of Hb from human whole blood and achieved satisfactory results by assaying with sodium dodecyl sulfate polyacrylamide gel electrophoresis.
ISSN	09476539
e-ISSN	15213765
Journal	Chemistry - A European Journal
Issue Number	17
Volume Number	17
Language	English
Publisher	Wiley-VCH;ChemPubSoc Europe
Publisher Date	2011-04-18
Publisher Place	Germany
Access Restriction	Open
Subject Keyword	Graphite Chemistry Hemoglobins Oxides Serum Albumin Silicon Dioxide Adsorption Circular Dichroism Electrophoresis, Polyacrylamide Gel Isolation & Purification Hydrogels Hydrogen-Ion Concentration Molecular Structure Spectrophotometry, Ultraviolet Spectroscopy, Fourier Transform Infrared Research Support, Non-U.S. Gov't
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Catalysis

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