NDLI: Simultaneous extraction, separation and purification of microbial genomic DNA and total RNA from acidic habitat samples

Please wait, while we are loading the content...

Characterization of multiple constituents in rat plasma after oral administration of Shengmai San using ultra-performance liquid chromatography coupled with electrospray ionization/quadrupole-time-of-flight high-definition mass spectrometry

A colorimetric assay of dopamine utilizing melamine modified gold nanoparticle probes

Disassembly of gold nanoparticle dimers for colorimetric detection of ochratoxin A

UPLC-Q-TOF-MS/MS fingerprinting for rapid identification of the chemical constituents of Ermiao Wan

Separation of asparagine, valine and tetraethylammonium ions overlapping in an ion mobility spectrum by clustering with methanol introduced as a modifier into the buffer gas

Profiling phenolic glycosides in Populus deltoides and Populus grandidentata by leaf spray ionization tandem mass spectrometry

Hyphenation of single-drop microextraction with laser-induced breakdown spectrometry for trace analysis in liquid samples: a viability study

Integrated hybrid polystyrene–polydimethylsiloxane device for monitoring cellular release with microchip electrophoresis and electrochemical detection

Electrochemical determination of luteolin in Chrysanthemum using multi-walled carbon nanotubes–ionic liquid composite electrode

Mesoporous carbon reinforced hollow fiber liquid-phase microextraction for the enrichment of phenylurea herbicides followed by their determination with high performance liquid chromatography

Simultaneous extraction, separation and purification of microbial genomic DNA and total RNA from acidic habitat samples

Metal organic framework MIL-101 coated fiber for headspace solid phase microextraction of volatile aromatic compounds

Sensitive colorimetric detection of melamine with 1,4-dithiothreitol modified gold nanoparticles

A durable solid contact sulfide sensor based on a ceric acrylohydrazide ionophore attached to polyacrylamide with a nanomolar detection limit

A novel method for rapid discrimination of bulbus of Fritillaria by using electronic nose and electronic tongue technology

Simple and useful method for determination of inorganic selenium species in real samples based on UV-VIS spectroscopy in a micellar medium

Analysis of different Flos Chrysanthemum tea samples with the use of two-dimensional chromatographic fingerprints, which were interpreted by different multivariate methods

A terminal protection system for the detection of adenosine triphosphate via enzyme-assisted signal amplification

Determination of alkaloids in Rhizoma coptidis by capillary electrophoresis with acidic potassium permanganate chemiluminescene detection

Evaluation of dispersive and cartridge SPE clean-up procedures using the modified QuEChERS method for the analysis of pesticides in strawberries

Photoelectrochemical glucose biosensor in flow injection analysis system based on glucose dehydrogenase immobilized on poly-hematoxylin modified glassy carbon electrode

Sensitive determination of domoic acid in mussel tissue using dansyl chloride derivatization and liquid chromatography-mass spectrometry

Evaluation of three types of protein extraction methods for tetraploid black locust (Robinia pseudoacacia L.) phloem tissue proteome analysis by two-dimensional electrophoresis

Successive detection of glucose and bio-copper in human serum based on a multiplex biosensor of gold nanorods

Electrochemical investigation and stripping voltammetric determination of captopril at CuO nanoparticles/multi-wall carbon nanotube nanocomposite electrode in tablet and urine samples

Fast and sensitive determination of sulfur dioxide in herbal medicines by microchip-based field asymmetric-wave ion mobility spectrometry

Determination of fluoroquinolone drugs in meat by ionic-liquid-based dispersive liquid–liquid microextraction-high performance liquid chromatography

Electrochemical evaluation of a boron-doped diamond electrode for simultaneous determination of an antihypertensive ternary mixture of amlodipine, hydrochlorothiazide and valsartan in pharmaceuticals

Diffuse reflectance FTIR database for the interpretation of the spectra obtained with a handheld device on built heritage materials

Determination of polycyclic aromatic hydrocarbons in water samples by hollow fiber extraction coupled with GC-MS

Magnetic retrieval of ionic liquid formed during in situ metathesis dispersive liquid–liquid microextraction–preconcentration of selected endocrine disrupting phenols from an enlarged sample volume

Enhanced gas-sensing performance of one-pot-synthesized Pt/CdIn₂O₄ composites with controlled morphologies

Supercritical fluid (CO₂) chromatography for quantitative determination of selected cancer therapeutic drugs in the presence of potential impurities

Determination of eight isoflavones in Radix Puerariae by capillary zone electrophoresis with an ionic liquid as an additive

A thermal desorption ion mobility spectrometer for the measurement of anticoagulant rodenticide diphacinone in beverages via in situ acid-assisted conversion

Colourimetric detection of Ag(i) ions using dCTP-stabilised gold nanoparticles

Using ionic liquid combined with HPLC-DAD to analyze semi-permanent hair dyes in commercial formulations

Extraction of fatty compounds from fingerprints for GCMS analysis

Glycine-functionalized carbon quantum dots as chemiluminescence sensitization for detection of m-phenylenediamine

Hollow fiber supported ionic liquid membrane microextraction for speciation of mercury by high-performance liquid chromatography-inductively coupled plasma mass spectrometry

Electroanalysis of nicotine at an electroreduced carboxylated graphene modified glassy carbon electrode

In situ growth of Fe₃O₄ nanoparticles for dispersive magnetic micro-solid phase extraction of cadmium followed by ETAAS detection

A pyrene-linked thiourea as a chemosensor for cations and simple fluorescent sensor for picric acid

A novel electrochemical sensor for capsaicin based on mesoporous cellular foams

Enhancement of the colorimetric response of enzymatic reactions by thermally evaporated plasmonic thin films: application to glial fibrillary acidic protein

A low-cost forward and reverse blood typing device—a blood sample is all you need to perform an assay

A new liquid–liquid microextraction method by ultrasound assisted salting-out for determination of triazole pesticides in water samples coupled by gas chromatography-mass spectrometry

Research on the detection of SF₆ decomposition products based on non-resonant photoacoustic spectroscopy

Exploring the electrical wiring of screen-printed configurations utilised in electroanalysis

A novel strategy to determine As, Cr, Hg and V in drinking water by ICP-MS/MS

Back cover

Analytical Methods Vol -6

Analytical Methods Vol -5

Analytical Methods Vol -4

Analytical Methods Vol -3

Analytical Methods Vol -2

Analytical Methods Vol -1

Simultaneous extraction, separation and purification of microbial genomic DNA and total RNA from acidic habitat samples

Content Provider	Royal Society of Chemistry (RSC)
Author	Liu, Xinxing Yun, Hui Dong, Haigang Xie, Jianping Zhao, Wenya Wang, Guohua Qiu, Guanzhou
Copyright Year	2015
Abstract	Although many studies have focused on DNA and RNA extraction, only a few have focused on the simultaneous extraction of high quality DNA and RNA from environmental samples, especially for acidic habitat samples. In this study, a widely used DNA extraction method (Zhou, J. Z., M. A. Bruns, and J. M. Tiedje 1996) was optimised for the simultaneous extraction of crude nucleic acid (including DNA and RNA), and a LiCl salt-based step-by-step precipitation method was also optimised for the separation of DNA and RNA. The results showed that nucleic acid extraction buffer (pH 7.0) containing piperazine-1,4-bis(2-ethanesulfonic acid) (PIPES) salt could effectively extract crude nucleic acid from acidic habitat samples, and the optimised LiCl salt-based step-by-step precipitation method could separate the nucleic acid into high quality DNA and RNA. The results also showed that the obtained DNA and RNA could be used for downstream molecular analysis. This study developed a useful method for the simultaneous extraction and separation of DNA and RNA from acidic habitat samples. This method has a great potential as a standard method to obtain high quality nucleic acid from biohydrometallurgy processes and extreme environmental samples.
Starting Page	909
Ending Page	917
Page Count	9
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	7
Issue Number	3
Journal	Analytical Methods
DOI	10.1039/c4ay01608d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Tiedje DNA extraction Zhou dynasty Acid RNA Lithium chloride Biohydrometallurgy RNA extraction Piperazine DNA PH Bruns Zhou
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

All in One High Quality Genomic DNA and Total RNA Extraction From Nematode Induced Galls for High Throughput Sequencing Purposes

Simultaneous extraction from bacterioplankton of total rna and dna suitable for quantitative structure and function (2002).

Development of a Nucleic Acid Extraction Procedure for Simultaneous Recovery of DNA and RNA from Diverse Microbes in Water

Transparent DNA/RNA Co-extraction Workflow Protocol Suitable for Inhibitor-Rich Environmental Samples That Focuses on Complete DNA Removal for Transcriptomic Analyses

Technologies of extraction and purification of microbial total DNA from a meadow soil

2-Octylaminopyridine assisted solvent extraction system for selective separation of palladium(ii) ion-pair complex from synthetic mixtures and real samples

On-chip real-time nucleic acid sequence-based amplification for RNA detection and amplification

Sequential purification of rna, dna and protein from a single sample using norgen's rna/dna/protein purification kit and comparison to a market competitor.

Nd, Am and Cm isotopic measurement after simultaneous separation in transmutation irradiated samples

Simultaneous extraction, separation and purification of microbial genomic DNA and total RNA from acidic habitat samples

Similar Documents

All in One High Quality Genomic DNA and Total RNA Extraction From Nematode Induced Galls for High Throughput Sequencing Purposes

Simultaneous extraction from bacterioplankton of total rna and dna suitable for quantitative structure and function (2002).

Development of a Nucleic Acid Extraction Procedure for Simultaneous Recovery of DNA and RNA from Diverse Microbes in Water

Transparent DNA/RNA Co-extraction Workflow Protocol Suitable for Inhibitor-Rich Environmental Samples That Focuses on Complete DNA Removal for Transcriptomic Analyses

Technologies of extraction and purification of microbial total DNA from a meadow soil

2-Octylaminopyridine assisted solvent extraction system for selective separation of palladium(ii) ion-pair complex from synthetic mixtures and real samples

On-chip real-time nucleic acid sequence-based amplification for RNA detection and amplification

Sequential purification of rna, dna and protein from a single sample using norgen's rna/dna/protein purification kit and comparison to a market competitor.

Nd, Am and Cm isotopic measurement after simultaneous separation in transmutation irradiated samples

Simultaneous extraction, separation and purification of microbial genomic DNA and total RNA from acidic habitat samples