NDLI: Study on Acridine Orange dimer as a new fluorescent probe for the determination of protein

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Content Provider	Royal Society of Chemistry (RSC)
Author	Hanxi, Shen Yunjing, Luo
Copyright Year	1999
Abstract	A nonfluorescent dimer of Acridine Orange is formed in situ in the presence of the anionic surfactant, sodium dodecyl sulfate (SDS). Proteins labeled with Acridine Orange dimer (AOAO) show a greatly enhanced fluorometric activity compared with that of AOAO. Based on this, a new, fast and sensitive fluorescent probe for the determination of proteins was developed. The linear range of this assay is 0.66–39.8 µg mL–1. For the detection of proteins in human serum, this method gave values close to that of the conventional Coomassie Brilliant Blue (CBB) method, but the sensitivity of the method is much superior to that of the CBB method. The detection limit for BSA was 0.08 µg mL–1.
Starting Page	135
Ending Page	137
Page Count	3
File Format	PDF
ISSN	13597337
Volume Number	36
Issue Number	4
Journal	Analytical Communications
DOI	10.1039/a900601j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Sodium dodecyl sulfate BSA Coomassie Brilliant Blue AOAO CBB Acridine Orange Protein dimer Surfactant
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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Study on Acridine Orange dimer as a new fluorescent probe for the determination of protein