Loading...
Please wait, while we are loading the content...
Similar Documents
Criticism of the use of coomassie brilliant blue g-250 for the quantitative determination of proteins (1980).
| Content Provider | CiteSeerX |
|---|---|
| Author | Werkman, E. M. A. Gorkom, W. H. Van Soons, B. J. |
| Abstract | Summary: The quantitative determination of proteins in biological fluids, using Coomassie Brilliant Blue G-250, was evaluated. Compared with the biuret method, the Coomassie Brilliant Blue G-250 method needs a much shorter time for analysis and has a greater sensitivity. The sensitivity of the dye for albumin is significantly greater than for globulins. The standard curves for the biuret method are more linear than those for the Coomassie Brilliant Blue G-250 method. The Coomassie Brilliant Blue G-250 method produces a precipitate, which sticks to the walls of the cuvet and results in an intolerable carry-over. Therefore, the use of the Coomassie Brilliant Blue G-250 method for the quantitative determination of proteins in urine and serum is not recommended. Kritik an der Verwendung von Coomassie Brilliant Blau G-250 zur quantitativ en Bestimmung von Proteinen Zusammenfassung: Die quantitative Bestimmung von Proteinen in biologischen Flüssigkeiten mit Coomassie Brilliant |
| File Format | |
| Publisher Date | 1980-01-01 |
| Access Restriction | Open |
| Subject Keyword | Quantitative Determination Coomassie Brilliant Blue G-250 Method Coomassie Brilliant Blue G-250 Biuret Method Standard Curve Biological Fluid Die Quantitative Bestimmung Von Proteinen Biologischen Fl Coomassie Brilliant Intolerable Carry-over |
| Content Type | Text |
