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Destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels with sodium chloride solutions
| Content Provider | CSIR - Central Food Technological Research Institute (CFTRI) |
|---|---|
| Author | Sreeramulu, G. Singh, N. K. |
| Date of Submission | 1995-01-01 |
| Abstract | A novel method for destaining of polyacrylamide gels, stained with Coomassie Brilliant Blue R-250, is described, based on the use of 0.5 M NaCl in water as the destainer, requiring only 2-3 h. Concentrated (> 2 M) or dilute (< 0.1 M) salt solutions were unsuitable. The method affords the advantage that expensive organic solvents, such as methanol, acetic acid or trichloroacetic acid, are not needed. Furthermore, salt destaining results in darker purple-blue protein bands as compared to the pale blue color with e.g. methanol/acetic acid destaining. |
| Starting Page | 362 |
| Ending Page | 365 |
| Page Count | 4 |
| File Format | |
| ISSN | 01730835 |
| Issue Number | 3 |
| Volume Number | 16 |
| Journal | Electrophoresis |
| Language | English |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Colours Destaining Sodium dodecyl sulfate polyacrylamide gel electrophoresis Wheat Prolamins |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry Biochemistry Clinical Biochemistry |
