NDLI: A second iron-regulatory system in yeast independent of Aft1p

Content Provider	PubMed Central
Author	Rutherford, Julian C. Jaron, Shulamit Ray, Esha Brown, Patrick O. Winge, Dennis R.
Copyright Year	2001
Abstract	Iron homeostasis in the yeast Saccharomyces cerevisiae is regulated at the transcriptional level by Aft1p, which activates the expression of its target genes in response to low-iron conditions. The yeast genome contains a paralog of AFT1, which has been designated AFT2. To establish whether AFT1 and AFT2 have overlapping functions, a mutant containing a double aft1Δaft2Δ deletion was generated. Growth assays established that the single aft2Δ strain exhibited no iron-dependent phenotype. However, the double-mutant aft1Δaft2Δ strain was more sensitive to low-iron growth conditions than the single-mutant aft1Δ strain. A mutant allele of AFT2 (AFT2-1 up ), or overexpression of the wild-type AFT2 gene, led to partial complementation of the respiratory-deficient phenotype of the aft1Δ strain. The AFT2-1 up allele also increased the uptake of 59Fe in an aft1Δ strain. DNA microarrays were used to identify genes regulated by AFT2. Some of the AFT2-regulated genes are known to be regulated by Aft1p; however, AFT2-1 up -dependent activation was independent of Aft1p. The kinetics of induction of two genes activated by the AFT2-1 up allele are consistent with Aft2p acting as a direct transcriptional factor. Truncated forms of Aft1p and Aft2p bound to a DNA duplex containing the Aft1p binding site in vitro. The wild-type allele of AFT2 activated transcription in response to growth under low-iron conditions. Together, these data suggest that yeast has a second regulatory pathway for the iron regulon, with AFT1 and AFT2 playing partially redundant roles.
Related Links	http://dx.doi.org/10.1073/pnas.261381198
Ending Page	14327
Page Count	6
Starting Page	14322
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	25
Volume Number	98
Language	English
Publisher	The National Academy of Sciences
Publisher Date	2001-12-04
Access Restriction	Open
Rights Holder	The National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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