NDLI: The yeast mutant vps5Δ affected in the recycling of Golgi membrane proteins displays an enhanced vacuolar Mg2+/H+ exchange activity

Content Provider	PubMed Central
Author	Gilles, Borrelly Boyer, Jean-christophe Touraine, Brigitte Wojciech, Szponarski Rambier, Michèle Gibrat, Rémy
Copyright Year	2001
Abstract	Growth of the yeast vacuolar protein-sorting mutant vps5Δ affected in the endosome-to-Golgi retromer complex was more sensitive to Mg2+-limiting conditions than was the growth of the wild-type (WT) strain. This sensitivity was enhanced at acidic pH. The vps5Δ strain was also sensitive to Al3+, known to inhibit Mg2+ uptake in yeast cells. In contrast, it was found to be resistant to Ni2+ and Co2+, two cytotoxic analogs of Mg2+. Resistance to Ni2+ did not seem to result from the alteration of plasma-membrane transport properties because mutant and WT cells displayed similar Ni2+ uptake. After plasma-membrane permeabilization, intracellular Ni2+ uptake in vps5Δ cells was 3-fold higher than in WT cells, which is consistent with the implication of the vacuole in the observed phenotypes. In reconstituted vacuolar vesicles prepared from vps5Δ, the rates of H+ exchange with Ni2+, Co2+, and Mg2+ were increased (relative to WT) by 170%, 130%, and 50%, respectively. The rates of H+ exchange with Ca2+, Cd2+, and K+ were similar in both strains, as were α-mannosidase and H+-ATPase activities, and SDS/PAGE patterns of vacuolar proteins. Among 14 other vacuolar protein-sorting mutants tested, only the 8 mutants affected in the recycling of trans-Golgi network membrane proteins shared the same Ni2+ resistance phenotype as vps5Δ. It is proposed that a trans-Golgi network Mg2+/H+ exchanger, mislocalized to vps5Δ vacuole, could be responsible for the phenotypes observed in vivo and in vitro.
Related Links	http://dx.doi.org/10.1073/pnas.161215198
Ending Page	9665
Page Count	6
Starting Page	9660
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	17
Volume Number	98
Language	English
Publisher	National Academy of Sciences
Publisher Date	2001-08-14
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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The yeast mutant vps5delta affected in the recycling of Golgi membrane proteins displays an enhanced vacuolar Mg2+/H+ exchange activity

The yeast mutant vps5Δ affected in the recycling of Golgi membrane proteins displays an enhanced vacuolar $Mg^{2+}/H^{+}$ exchange activity

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The yeast mutant vps5Δ affected in the recycling of Golgi membrane proteins displays an enhanced vacuolar Mg2+/H+ exchange activity

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The yeast mutant vps5delta affected in the recycling of Golgi membrane proteins displays an enhanced vacuolar Mg2+/H+ exchange activity

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