NDLI: Molecular structure of double-minute chromosomes bearing amplified copies of the epidermal growth factor receptor gene in gliomas

Content Provider	PubMed Central
Author	Vogt, Nicolas Lefèvre, Sandrine-hélène Apiou, Françoise Dutrillaux, Anne-marie Andrej, Cör Pascal, Leuraud Poupon, Marie-france Bernard, Dutrillaux Michelle, Debatisse Bernard, Malfoy
Copyright Year	2004
Abstract	Amplification of the epidermal growth factor receptor gene on double minutes is recurrently observed in cells of advanced gliomas, but the structure of these extrachromosomal circular DNA molecules and the mechanisms responsible for their formation are still poorly understood. By using quantitative PCR and chromosome walking, we investigated the genetic content and the organization of the repeats in the double minutes of seven gliomas. It was established that all of the amplicons of a given tumor derive from a single founding extrachromosomal DNA molecule. In each of these gliomas, the founding molecule was generated by a simple event that circularizes a chromosome fragment overlapping the epidermal growth factor receptor gene. In all cases, the fusion of the two ends of this initial amplicon resulted from microhomology-based nonhomologous end-joining. Furthermore, the corresponding chromosomal loci were not rearranged, which strongly suggests that a postreplicative event was responsible for the formation of each of these initial amplicons.
Related Links	http://dx.doi.org/10.1073/pnas.0402979101
Ending Page	11373
Page Count	6
Starting Page	11368
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	31
Volume Number	101
Language	English
Publisher	National Academy of Sciences
Publisher Date	2004-08-03
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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