NDLI: Structural Variabilities in β-Lactamase (blaA) of DifferentBiovars of Yersinia enterocolitica: Implications for β-Lactam Antibioticand β-Lactamase Inhibitor Susceptibilities

Content Provider	PubMed Central
Author	Singhal, Neelja Srivastava, Abhishikha Kumar, Manish Virdi, Jugsharan Singh
Editor	Salsbury , Jr,Freddie
Copyright Year	2015
Abstract	Yersiniosis caused by Yersinia enterocolitica has been reportedfrom all continents. The bacterial species is divided into more than fiftyserovars and six biovars viz. 1A, 1B, 2, 3, 4 and 5 whichdiffer in geographical distribution, ecological niches and pathogenicity. MostY.enterocolitica strains harborchromosomal genes for two β-lactamases, blaA an Amblerclass A penicillinase and blaB an Ambler class C induciblecephalosporinase. In the present study, susceptibility to b-lactam antibioticsand β-lactamase inhibitor was studied for Y.enterocolitica strains of biovars 1A, 1B, 2 and 4. Weobserved that β-lactamases were expressed differentially among strains ofdifferent biovars. To understand the molecular mechanisms underlying suchdifferential expression, the sequences of genes and promoters ofblaA were compared. Also, the variants of blaA present indifferent biovars were modeled and docked with amoxicillin and clavulanic acid.The mRNA secondary structures of blaA variants were also predictedin-silico. Our findings indicated that neither variationsin the promoter regions, nor the secondary structures of mRNA contributed tohigher/lower expression of blaA in different biovars. Analysis of H-bondingresidues of blaA variants with amoxicillin and clavulanic acid revealed that ifamino acid residues of a β-lactamase interacting with amoxicillin and theclavulanic acid were similar, clavulanic acid was effective in engaging theenzyme, accounting for a significant reduction in MIC ofamoxicillin-clavulanate. This finding might aid in designing betterβ-lactamase inhibitors with improved efficiencies in future.
Related Links	http://dx.doi.org/10.1371/journal.pone.0123564
Starting Page	123564
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	4
Volume Number	10
Language	English
Publisher	Public Library of Science
Publisher Date	2015-04-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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