NDLI: Equid Herpesvirus Type 1 Activates Platelets

Content Provider	PubMed Central
Author	Tracy, Stokol Yeo, Wee Ming Burnett, Deborah Deangelis, Nicole Huang, Teng Nikolaus, Osterrieder Catalfamo, James
Editor	Stewart, James P.
Copyright Year	2015
Abstract	Equid herpesvirus type 1 (EHV-1) causes outbreaks of abortion and neurological disease in horses. One of the main causes of these clinical syndromes is thrombosis in placental and spinal cord vessels, however the mechanism for thrombus formation is unknown. Platelets form part of the thrombus and amplify and propagate thrombin generation. Here, we tested the hypothesis that EHV-1 activates platelets. We found that two EHV-1 strains, RacL11 and Ab4 at 0.5 or higher plaque forming unit/cell, activate platelets within 10 minutes, causing α-granule secretion (surface P-selectin expression) and platelet microvesiculation (increased small events double positive for CD41 and Annexin V). Microvesiculation was more pronounced with the RacL11 strain. Virus-induced P-selectin expression required plasma and 1.0 mM exogenous calcium. P-selectin expression was abolished and microvesiculation was significantly reduced in factor VII- or X-deficient human plasma. Both P-selectin expression and microvesiculation were re-established in factor VII-deficient human plasma with added purified human factor VIIa (1 nM). A glycoprotein C-deficient mutant of the Ab4 strain activated platelets as effectively as non-mutated Ab4. P-selectin expression was abolished and microvesiculation was significantly reduced by preincubation of virus with a goat polyclonal anti-rabbit tissue factor antibody. Infectious virus could be retrieved from washed EHV-1-exposed platelets, suggesting a direct platelet-virus interaction. Our results indicate that EHV-1 activates equine platelets and that α-granule secretion is a consequence of virus-associated tissue factor triggering factor X activation and thrombin generation. Microvesiculation was only partly tissue factor and thrombin-dependent, suggesting the virus causes microvesiculation through other mechanisms, potentially through direct binding. These findings suggest that EHV-1-induced platelet activation could contribute to the thrombosis that occurs in clinically infected horses and provides a new mechanism by which viruses activate hemostasis.
Related Links	http://dx.doi.org/10.1371/journal.pone.0122640
Starting Page	122640
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	4
Volume Number	10
Language	English
Publisher	Public Library of Science
Publisher Date	2015-04-23
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Failure to detect equid herpesvirus types 1 and 4 DNA in placentae and healthy new-born Thoroughbred foals

Editorial: Current Research in Equid Herpesvirus Type-1 (EHV-1)

Innate immunity during Equid herpesvirus 1 (EHV-1) infection.

Genetic characterization of equid herpesvirus type 1 from cases of abortion in Poland

First identification and isolation of equine herpesvirus type 1 in aborted fetal lung tissues of donkeys

Measurement of neutralizing antibody to equid herpesvirus 1 by single radial hemolysis.

Genome‐wide association study for host genetic factors associated with equine herpesvirus type‐1 induced myeloencephalopathy

Genetic restriction of cytolysis during equid herpesvirus 1 subtype 2 infection.

Screening of potential antiviral molecules against equid herpesvirus-1 using cellular impedance measurement: dataset of 2,891 compounds

Equid Herpesvirus Type 1 Activates Platelets

Similar Documents

Failure to detect equid herpesvirus types 1 and 4 DNA in placentae and healthy new-born Thoroughbred foals

Editorial: Current Research in Equid Herpesvirus Type-1 (EHV-1)

Innate immunity during Equid herpesvirus 1 (EHV-1) infection.

Genetic characterization of equid herpesvirus type 1 from cases of abortion in Poland

First identification and isolation of equine herpesvirus type 1 in aborted fetal lung tissues of donkeys

Measurement of neutralizing antibody to equid herpesvirus 1 by single radial hemolysis.

Genome‐wide association study for host genetic factors associated with equine herpesvirus type‐1 induced myeloencephalopathy

Genetic restriction of cytolysis during equid herpesvirus 1 subtype 2 infection.

Screening of potential antiviral molecules against equid herpesvirus-1 using cellular impedance measurement: dataset of 2,891 compounds

Equid Herpesvirus Type 1 Activates Platelets