NDLI: Interferon-γ Suppresses Intestinal Epithelial Aquaporin-1Expression via Janus Kinase and STAT3 Activation

Content Provider	PubMed Central
Author	Dicay, Michael S. Hirota, Christina L. Ronaghan, Natalie J. Peplowski, Michael A. Zaheer, Raza S. Carati, Colin A. Macnaughton, Wallace K.
Editor	Sly, Laura May
Copyright Year	2015
Abstract	Inflammatory bowel diseases are associated with dysregulated electrolyte andwater transport and resultant diarrhea. Aquaporins are transmembrane proteinsthat function as water channels in intestinal epithelial cells. We investigatedthe effect of the inflammatory cytokine, interferon-γ, which is a majorplayer in inflammatory bowel diseases, on aquaporin-1 expression in a mousecolonic epithelial cell line, CMT93. CMT93 monolayers were exposed to 10 ng/mLinterferon-γ and aquaporin-1 mRNA and protein expressions were measuredby real-time PCR and western blot, respectively. In other experiments, CMT93cells were pretreated with inhibitors or were transfected with siRNA to blockthe effects of Janus kinases, STATs 1 and 3, or interferon regulatory factor 2,prior to treatment with interferon-γ. Interferon-γ decreasedaquaporin-1 expression in mouse intestinal epithelial cells in a manner that didnot depend on the classical STAT1/JAK2/IRF-1 pathway, but rather, on analternate Janus kinase (likely JAK1) as well as on STAT3. The pro-inflammatorycytokine, interferon-γ may contribute to diarrhea associated withintestinal inflammation in part through regulation of the epithelial aquaporin-1water channel via a non-classical JAK/STAT receptor signalling pathway.
Related Links	http://dx.doi.org/10.1371/journal.pone.0118713
Starting Page	118713
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	3
Volume Number	10
Language	English
Publisher	Public Library of Science
Publisher Date	2015-03-20
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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