NDLI: H-Ferritin-Regulated MicroRNAs Modulate Gene Expression in K562 Cells

Content Provider	PubMed Central
Author	Biamonte, Flavia Zolea, Fabiana Bisognin, Andrea Sanzo, Maddalena Di Claudia, Saccoman Scumaci, Domenica Aversa, Ilenia Panebianco, Mariafranca Faniello, Maria Concetta Bortoluzzi, Stefania Cuda, Giovanni Costanzo, Francesco
Editor	Kostas, Pantopoulos
Copyright Year	2015
Abstract	In a previous study, we showed that the silencing of the heavy subunit (FHC) offerritin, the central iron storage molecule in the cell, is accompanied by a modification in global gene expression. In this work, we explored whether different FHC amounts might modulate miRNA expression levels in K562 cells and studied the impact of miRNAs in gene expression profile modifications. To this aim, we performed a miRNA-mRNA integrative analysis in K562 silenced for FHC (K562shFHC) comparing it with K562 transduced with scrambled RNA (K562shRNA). Four miRNAs, namely hsa-let-7g, hsa-let-7f, hsa-let-7i and hsa-miR-125b, were significantly up-regulated in silenced cells. The remarkable down-regulation of these miRNAs, following FHC expression rescue, supports a specific relation between FHC silencing and miRNA-modulation. The integration of target predictions with miRNA and gene expression profiles led to the identification of a regulatory network which includes the miRNAs up-regulated by FHC silencing, as well as91 down-regulated putative target genes. These genes were further classified in 9 networks; the highest scoring network, “Cell Death and Survival, Hematological System Development and Function, Hematopoiesis”, is composed by 18 focus molecules including RAF1 and ERK1/2. We confirmed that, following FHC silencing, ERK1/2 phosphorylation is severely impaired and that RAF1 mRNA is significantly down-regulated. Taken all together, our data indicate that, in our experimental model, FHC silencing may affect RAF1/pERK1/2 levels through the modulation of a specific set of miRNAs and add new insights in to the relationship among iron homeostasis and miRNAs.
Related Links	http://dx.doi.org/10.1371/journal.pone.0122105
Starting Page	122105
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	3
Volume Number	10
Language	English
Publisher	Public Library of Science
Publisher Date	2015-03-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Comprehensive Expression Profiling and Functional Network Analysis of p53-Regulated MicroRNAs in HepG2 Cells Treated with Doxorubicin

FTH1P3, a Novel H-Ferritin Pseudogene Transcriptionally Active, Is Ubiquitously Expressed and Regulated during Cell Differentiation

Proanthocyanidins Modulate MicroRNA Expression in Human HepG2 Cells

Topoisomerase Inhibitors Modulate Gene Expression of B-Cell Translocation Gene 2 and Prostate Specific Antigen in Prostate Carcinoma Cells

Environmental Alkylphenols Modulate Cytokine Expression in Plasmacytoid Dendritic Cells

Sarcoptes scabiei Mites Modulate Gene Expression in Human Skin Equivalents

Prediction of Associations between microRNAs and Gene Expression in Glioma Biology

Improved Expression Systems for Regulated Expression in Salmonella Infecting Eukaryotic Cells

An Optimized, Chemically Regulated Gene Expression System for Chlamydomonas

H-Ferritin-Regulated MicroRNAs Modulate Gene Expression in K562 Cells

Similar Documents

Comprehensive Expression Profiling and Functional Network Analysis of p53-Regulated MicroRNAs in HepG2 Cells Treated with Doxorubicin

FTH1P3, a Novel H-Ferritin Pseudogene Transcriptionally Active, Is Ubiquitously Expressed and Regulated during Cell Differentiation

Proanthocyanidins Modulate MicroRNA Expression in Human HepG2 Cells

Topoisomerase Inhibitors Modulate Gene Expression of B-Cell Translocation Gene 2 and Prostate Specific Antigen in Prostate Carcinoma Cells

Environmental Alkylphenols Modulate Cytokine Expression in Plasmacytoid Dendritic Cells

Sarcoptes scabiei Mites Modulate Gene Expression in Human Skin Equivalents

Prediction of Associations between microRNAs and Gene Expression in Glioma Biology

Improved Expression Systems for Regulated Expression in Salmonella Infecting Eukaryotic Cells

An Optimized, Chemically Regulated Gene Expression System for Chlamydomonas

H-Ferritin-Regulated MicroRNAs Modulate Gene Expression in K562 Cells