NDLI: Insights into the fate of the N-terminal amyloidogenic polypeptide of ApoA-I in cultured target cells

Content Provider	PubMed Central
Author	Angela, Arciello Marco, Nadia De Giudice, Rita Del Guglielmi, Fulvio Pucci, Piero Annalisa, Relini Monti, Daria Maria Piccoli, Renata
Copyright Year	2011
Abstract	Apolipoprotein A-I (ApoA-I) is an extracellular lipid acceptor, whose role in cholesterol efflux and high-density lipoprotein formation is mediated by ATP-binding cassette transporter A1 (ABCA1). Nevertheless, some ApoA-I variants are associated to systemic forms of amyloidosis, characterized by extracellular fibril deposition in peripheral organs. Heart amyloid fibrils were found to be mainly constituted by the 93-residue N-terminal fragment of ApoA-I, named [1–93]ApoA-I. In this paper, rat cardiomyoblasts were used as target cells to analyse binding, internalization and intracellular fate of the fibrillogenic polypeptide in comparison to full-length ApoA-I. We provide evidence that the polypeptide: (i) binds to specific sites on cell membrane (Kd= 5.90 ± 0.70 × 10−7 M), where it partially co-localizes with ABCA1, as also described for ApoA-I; (ii) is internalized mostly by chlatrin-mediated endocytosis and lipid rafts, whereas ApoA-I is internalized preferentially by chlatrin-coated pits and macropinocytosis and (iii) is rapidly degraded by proteasome and lysosomes, whereas ApoA-I partially co-localizes with recycling endosomes. Vice versa, amyloid fibrils, obtained by in vitro aggregation of [1–93]ApoA-I, were found to be unable to enter the cells. We propose that internalization and intracellular degradation of [1–93]ApoA-I may divert the polypeptide from amyloid fibril formation and contribute to the slow progression and late onset that characterize this pathology.
Related Links	http://dx.doi.org/10.1111/j.1582-4934.2011.01271.x
Ending Page	2663
Page Count	12
Starting Page	2652
File Format	PDF
ISSN	15824934
e-ISSN	15824934
Journal	Journal of Cellular and Molecular Medicine
Issue Number	12
Volume Number	15
Language	English
Publisher	Blackwell Publishing Ltd
Publisher Date	2011-12-01
Access Restriction	Open
Rights Holder	Blackwell Publishing Ltd
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Medicine

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