NDLI: Transcriptome Analysis of Resistant and Susceptible Alfalfa Cultivars Infected With Root-Knot Nematode Meloidogyne incognita

Content Provider	PubMed Central
Author	Postnikova, Olga A. Hult, Maria Shao, Jonathan Skantar, Andrea Nemchinov, Lev G.
Editor	Jones, John
Abstract	Nematodes are one of the major limiting factors in alfalfa production. Root-knot nematodes (RKN, Meloidogyne spp.) are widely distributed and economically important sedentary endoparasites of agricultural crops and they may inflict significant damage to alfalfa fields. As of today, no studies have been published on global gene expression profiling in alfalfa infected with RKN or any other plant parasitic nematode. Very little information is available about molecular mechanisms that contribute to pathogenesis and defense responses in alfalfa against these pests and specifically against RKN. In this work, we performed root transcriptome analysis of resistant (cv. Moapa 69) and susceptible (cv. Lahontan) alfalfa cultivars infected with RKN Meloidogyne incognita, widespread root-knot nematode species and a major pest worldwide. A total of 1,701,622,580 pair-end reads were generated on an Illumina Hi-Seq 2000 platform from the roots of both cultivars and assembled into 45,595 and 47,590 transcripts in cvs Moapa 69 and Lahontan, respectively. Bioinformatic analysis revealed a number of common and unique genes that were differentially expressed in susceptible and resistant lines as a result of nematode infection. Although the susceptible cultivar showed a more pronounced defense response to the infection, feeding sites were successfully established in its roots. Characteristically, basal gene expression levels under normal conditions differed between the two cultivars as well, which may confer advantage to one of the genotypes toward resistance to nematodes. Differentially expressed genes were subsequently assigned to known Gene Ontology categories to predict their functional roles and associated biological processes. Real-time PCR validated expression changes in genes arbitrarily selected for experimental confirmation. Candidate genes that contribute to protection against M. incognita in alfalfa were proposed and alfalfa-nematode interactions with respect to resistance are discussed.
Related Links	http://dx.doi.org/10.1371/journal.pone.0118269
Starting Page	118269
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	2
Volume Number	10
Language	English
Publisher	Public Library of Science
Publisher Date	2015-02-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Correction: Transcriptome Analysis of Resistant and Susceptible Alfalfa Cultivars Infected With Root-Knot Nematode Meloidogyne incognita

Biochemical Alterations in Resistant and Susceptible Tomato Cultivars Infected by Root Knot Nematode Meloidogyne Incognita.

Biochemical Changes in Resistant And Susceptible Cowpea Cultivars Infected By Root Knot Nematode, Meloidogyne Incognita

Screening of Germplasms and Biochemical Analysis of Resistant and Susceptible Cultivars if Gladiolus infected by Root Knot Nematode (Meloidogyne Incognita).

Screening of Germplasms and Biochenical Analysis of Resistant and Susceptible Cultivars of Cucumber Infected By Root-Knot Nematode (Meloidogyne Incognita.

Screening of germplasms and biochemical analysis of resistant and susceptible cultivars of cucumber infected by root-knot nematode (Meloidogyne incognita)

Screening of germplasms & biochemical analysis of resistant & susceptible cultivars of bittergourd infected by root knot nematode (Meloidogyne incognita)

Biochemical Variations in Resistant and Susceptible Brinjal Verieties Infected by Root Knot Nematode Meloidogyne Incognita

Biochemical Changes In Susceptible And Resistant Black Gram Cultivars Induced By Root-Knot Nematode, Meloidogyne Incognita

Transcriptome Analysis of Resistant and Susceptible Alfalfa Cultivars Infected With Root-Knot Nematode Meloidogyne incognita

Similar Documents

Correction: Transcriptome Analysis of Resistant and Susceptible Alfalfa Cultivars Infected With Root-Knot Nematode Meloidogyne incognita

Biochemical Alterations in Resistant and Susceptible Tomato Cultivars Infected by Root Knot Nematode Meloidogyne Incognita.

Biochemical Changes in Resistant And Susceptible Cowpea Cultivars Infected By Root Knot Nematode, Meloidogyne Incognita

Screening of Germplasms and Biochemical Analysis of Resistant and Susceptible Cultivars if Gladiolus infected by Root Knot Nematode (Meloidogyne Incognita).

Screening of Germplasms and Biochenical Analysis of Resistant and Susceptible Cultivars of Cucumber Infected By Root-Knot Nematode (Meloidogyne Incognita.

Screening of germplasms and biochemical analysis of resistant and susceptible cultivars of cucumber infected by root-knot nematode (Meloidogyne incognita)

Screening of germplasms & biochemical analysis of resistant & susceptible cultivars of bittergourd infected by root knot nematode (Meloidogyne incognita)

Biochemical Variations in Resistant and Susceptible Brinjal Verieties Infected by Root Knot Nematode Meloidogyne Incognita

Biochemical Changes In Susceptible And Resistant Black Gram Cultivars Induced By Root-Knot Nematode, Meloidogyne Incognita

Transcriptome Analysis of Resistant and Susceptible Alfalfa Cultivars Infected With Root-Knot Nematode Meloidogyne incognita