NDLI: Associations among Sebox and Other MEGs and Its Effects on Early Embryogenesis

Content Provider	PubMed Central
Author	Park, Min-woo Kim, Kyeoung-hwa Kim, Eun-young Lee, Su-yeon Ko, Jung-jae Lee, Kyung-ah
Editor	Sun, Qing-yuan
Copyright Year	2015
Abstract	In a previous report, we identified Sebox as a new candidate maternal effect gene that is essential for embryonic development and primarily impacts the two-cell (2C) stage. The present study was conducted to determine the mechanism of action for Sebox in this capacity, as shown by changes in the expression levels of other known MEG mRNAs after Sebox RNA interference (RNAi) in oocytes. Sebox-knockdown metaphase II (Mll) oocytes displayed normal morphology, but among the 23 MEGs monitored, 8 genes were upregulated, and 15 genes were unchanged. We hypothesized that the perturbed gene expression of these MEGs may cause the arrest of embryo development at the 2C stage and examined the expression of several marker genes for the degradation of maternal factors and zygotic genome activation. We found that some maternal mRNAs, c-mos, Gbx2, and Gdf9, were not fully degraded in Sebox-knockdown 2C embryos, and that several zygotic genome activation markers, Mt1a, Rpl23, Ube2a and Wee1, were not fully expressed in conjunction with diminished embryonic transcriptional activity. In addition, Sebox may be involved in the formation of the subcortical maternal complex through its regulation of the upstream regulator, Figla. Therefore, we concluded that Sebox is important in preparing oocytes for embryonic development by orchestrating the expression of other important MEGs.
Related Links	http://dx.doi.org/10.1371/journal.pone.0115050
Starting Page	115050
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	2
Volume Number	10
Language	English
Publisher	Public Library of Science
Publisher Date	2015-02-13
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Cell adhesion molecules in early chicken embryogenesis.

Paternal effects on early embryogenesis

Telomere length is reset during early mammalian embryogenesis

Sexual Dimorphism in the Early Embryogenesis in Zebra Finches

Mammalian DNA topoisomerase IIIα is essential in early embryogenesis

Alterations in chromatin structure during early sea urchin embryogenesis.

FtsHi4 Is Essential for Embryogenesis Due to Its Influence on Chloroplast Development in Arabidopsis

Linker histone variants control chromatin dynamics during early embryogenesis

Visualizing enveloping layer glycans during zebrafish early embryogenesis

Associations among Sebox and Other MEGs and Its Effects on Early Embryogenesis

Similar Documents

Cell adhesion molecules in early chicken embryogenesis.

Paternal effects on early embryogenesis

Telomere length is reset during early mammalian embryogenesis

Sexual Dimorphism in the Early Embryogenesis in Zebra Finches

Mammalian DNA topoisomerase IIIα is essential in early embryogenesis

Alterations in chromatin structure during early sea urchin embryogenesis.

FtsHi4 Is Essential for Embryogenesis Due to Its Influence on Chloroplast Development in Arabidopsis

Linker histone variants control chromatin dynamics during early embryogenesis

Visualizing enveloping layer glycans during zebrafish early embryogenesis

Associations among Sebox and Other MEGs and Its Effects on Early Embryogenesis