NDLI: A Stable and Reproducible Human Blood-Brain Barrier Model Derived from Hematopoietic Stem Cells

Content Provider	PubMed Central
Author	Romeo, Cecchelli Aday, Sezin Sevin, Emmanuel Almeida, Catarina Culot, Maxime Lucie, Dehouck Caroline, Coisne Engelhardt, Britta Dehouck, Marie-pierre Ferreira, Lino
Editor	Klein, Robyn
Copyright Year	2014
Abstract	The human blood brain barrier (BBB) is a selective barrier formed by human brain endothelial cells (hBECs), which is important to ensure adequate neuronal function and protect the central nervous system (CNS) from disease. The development of human in vitro BBB models is thus of utmost importance for drug discovery programs related to CNS diseases. Here, we describe a method to generate a human BBB model using cord blood-derived hematopoietic stem cells. The cells were initially differentiated into ECs followed by the induction of BBB properties by co-culture with pericytes. The brain-like endothelial cells (BLECs) express tight junctions and transporters typically observed in brain endothelium and maintain expression of most in vivo BBB properties for at least 20 days. The model is very reproducible since it can be generated from stem cells isolated from different donors and in different laboratories, and could be used to predict CNS distribution of compounds in human. Finally, we provide evidence that Wnt/β-catenin signaling pathway mediates in part the BBB inductive properties of pericytes.
Related Links	http://dx.doi.org/10.1371/journal.pone.0099733
Starting Page	99733
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	6
Volume Number	9
Language	English
Publisher	Public Library of Science
Publisher Date	2014-06-17
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A retinoic acid-enhanced, multicellular human blood-brain barrier model derived from stem cell sources

Contribution of brain pericytes in blood–brain barrier formation and maintenance: a transcriptomic study of cocultured human endothelial cells derived from hematopoietic stem cells

Hematopoietic colony-forming cells derived from human embryonic stem cells

A retinoic acid-enhanced, multicellular human blood-brain barrier model derived from stem cell sources

Quantitative Targeted Absolute Proteomics for Better Characterization of an In Vitro Human Blood–Brain Barrier Model Derived from Hematopoietic Stem Cells

Human ES-derived MSCs correct TNF-α-mediated alterations in a blood–brain barrier model

Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells as a Cellular Model to Study Neisseria meningitidis Infection

A human pluripotent stem cell-derived in vitro model of the blood–brain barrier in cerebral malaria

Wnt signaling mediates acquisition of blood-brain barrier properties in naïve endothelium derived from human pluripotent stem cells

A Stable and Reproducible Human Blood-Brain Barrier Model Derived from Hematopoietic Stem Cells

Similar Documents

A retinoic acid-enhanced, multicellular human blood-brain barrier model derived from stem cell sources

Contribution of brain pericytes in blood–brain barrier formation and maintenance: a transcriptomic study of cocultured human endothelial cells derived from hematopoietic stem cells

Hematopoietic colony-forming cells derived from human embryonic stem cells

A retinoic acid-enhanced, multicellular human blood-brain barrier model derived from stem cell sources

Quantitative Targeted Absolute Proteomics for Better Characterization of an In Vitro Human Blood–Brain Barrier Model Derived from Hematopoietic Stem Cells

Human ES-derived MSCs correct TNF-α-mediated alterations in a blood–brain barrier model

Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells as a Cellular Model to Study Neisseria meningitidis Infection

A human pluripotent stem cell-derived in vitro model of the blood–brain barrier in cerebral malaria

Wnt signaling mediates acquisition of blood-brain barrier properties in naïve endothelium derived from human pluripotent stem cells

A Stable and Reproducible Human Blood-Brain Barrier Model Derived from Hematopoietic Stem Cells