NDLI: Exopolysaccharide (EPS) Synthesis by Oenococcus oeni: From Genes to Phenotypes

Content Provider	PubMed Central
Author	Maria, Dimopoulou Vuillemin, Marlène Hugo, Campbell-sills Lucas, Patrick M. Ballestra, Patricia Miot-sertier, Cécile Favier, Marion Coulon, Joana Moine, Virginie Thierry, Doco Roques, Maryline Williams, Pascale Melina, Petrel Gontier, Etienne Moulis, Claire Magali, Remaud-simeon Marguerite, Dols-lafargue
Editor	Benoit, Foligne
Copyright Year	2014
Abstract	Oenococcus oeni is the bacterial species which drives malolactic fermentation in wine. The analysis of 50 genomic sequences of O. oeni (14 already available and 36 newly sequenced ones) provided an inventory of the genes potentially involved in exopolysaccharide (EPS) biosynthesis. The loci identified are: two gene clusters named eps1 and eps2, three isolated glycoside-hydrolase genes named dsrO, dsrV and levO, and three isolated glycosyltransferase genes named gtf, it3, it4. The isolated genes were present or absent depending on the strain and the eps gene clusters composition diverged from one strain to another. The soluble and capsular EPS production capacity of several strains was examined after growth in different culture media and the EPS structure was determined. Genotype to phenotype correlations showed that several EPS biosynthetic pathways were active and complementary in O. oeni. Can be distinguished: (i) a Wzy -dependent synthetic pathway, allowing the production of heteropolysaccharides made of glucose, galactose and rhamnose, mainly in a capsular form, (ii) a glucan synthase pathway (Gtf), involved in β-glucan synthesis in a free and a cell-associated form, giving a ropy phenotype to growth media and (iii) homopolysaccharide synthesis from sucrose (α-glucan or β-fructan) by glycoside-hydrolases of the GH70 and GH68 families. The eps gene distribution on the phylogenetic tree was examined. Fifty out of 50 studied genomes possessed several genes dedicated to EPS metabolism. This suggests that these polymers are important for the adaptation of O. oeni to its specific ecological niche, wine and possibly contribute to the technological performance of malolactic starters.
Related Links	http://dx.doi.org/10.1371/journal.pone.0098898
Starting Page	98898
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	6
Volume Number	9
Language	English
Publisher	Public Library of Science
Publisher Date	2014-06-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Exopolysaccharide (EPS) synthesis by oenococcus oeni: from genes to phenotypes

Identification of pOENI-1 and Related Plasmids in Oenococcus oeni Strains Performing the Malolactic Fermentation in Wine

Molecular Cloning, Expression and Characterization of Oenococcus oeni Priming Glycosyltransferases

Improving acid tolerance in Oenococcus oeni by Adaptive Evolution

Interactions between the FTO and GNB3 Genes Contribute to Varied Clinical Phenotypes in Hypertension

An Effective Method to Identify Heritable Components from Multivariate Phenotypes

Integrating Diverse Types of Genomic Data to Identify Genes that Underlie Adverse Pregnancy Phenotypes

FrzS Regulates Social Motility in Myxococcus xanthus by Controlling Exopolysaccharide Production

Molecular Mechanisms of Exopolysaccharide from Aphanothece halaphytica (EPSAH) Induced Apoptosis in HeLa Cells

Exopolysaccharide (EPS) Synthesis by Oenococcus oeni: From Genes to Phenotypes

Similar Documents

Exopolysaccharide (EPS) synthesis by oenococcus oeni: from genes to phenotypes

Identification of pOENI-1 and Related Plasmids in Oenococcus oeni Strains Performing the Malolactic Fermentation in Wine

Molecular Cloning, Expression and Characterization of Oenococcus oeni Priming Glycosyltransferases

Improving acid tolerance in Oenococcus oeni by Adaptive Evolution

Interactions between the FTO and GNB3 Genes Contribute to Varied Clinical Phenotypes in Hypertension

An Effective Method to Identify Heritable Components from Multivariate Phenotypes

Integrating Diverse Types of Genomic Data to Identify Genes that Underlie Adverse Pregnancy Phenotypes

FrzS Regulates Social Motility in Myxococcus xanthus by Controlling Exopolysaccharide Production

Molecular Mechanisms of Exopolysaccharide from Aphanothece halaphytica (EPSAH) Induced Apoptosis in HeLa Cells

Exopolysaccharide (EPS) Synthesis by Oenococcus oeni: From Genes to Phenotypes