NDLI: Multiplex genomic profiling of non–small cell lung cancers from the LETS phase III trial of first-line S-1/carboplatin versus paclitaxel/carboplatin: results of a West Japan Oncology Group study

Content Provider	PubMed Central
Author	Okamoto, Isamu Sakai, Kazuko Morita, Satoshi Yoshioka, Hiroshige Kaneda, Hiroyasu Takeda, Koji Hirashima, Tomonori Kogure, Yoshihito Kimura, Tatsuo Takahashi, Toshiaki Atagi, Shinji Seto, Takashi Sawa, Toshiyuki Yamamoto, Masashi Satouchi, Miyako Okuno, Motoyasu Nagase, Seisuke Takayama, Koichi Tomii, Keisuke Maeda, Tadashi Oizumi, Satoshi Fujii, Shinji Akashi, Yusaku Nishino, Kazumi Ebi, Noriyuki Nakagawa, Kazuhiko Nakanishi, Yoichi Nishio, Kazuto
Copyright Year	2014
Abstract	Archival formalin-fixed, paraffin-embedded (FFPE) tumor specimens were collected from advanced NSCLC patients enrolled in LETS phase III trial comparing first-line S-1/carboplatin with paclitaxel/carboplatin and subjected to multiplex genotyping for 214 somatic hotspot mutations in 26 genes (LungCarta Panel) and 20 major variants of ALK, RET, and ROS1 fusion genes (LungFusion Panel) with the Sequenom MassARRAY platform. MET amplification was evaluated by fluorescence in situ hybridization. A somatic mutation in at least one gene was identified in 48% of non–squamous cell carcinoma and 45% of squamous cell carcinoma specimens, with EGFR (17%), TP53 (11%), STK11 (9.8%), MET (7.6%), and KRAS (6.2%). Mutations in EGFR or KRAS were associated with a longer or shorter median overall survival, respectively. The LungFusion Panel identified ALK fusions in six cases (2.5%), ROS1 fusions in five cases (2.1%), and a RET fusion in one case (0.4%), with these three types of rearrangement being mutually exclusive. Nine (3.9%) of 229 patients were found to be positive for de novo MET amplification. This first multiplex genotyping of NSCLC associated with a phase III trial shows that MassARRAY-based genetic testing for somatic mutations and fusion genes performs well with nucleic acid derived from FFPE specimens of NSCLC tissue.
Related Links	http://dx.doi.org/10.18632/oncotarget.1906
Ending Page	2304
Page Count	12
Starting Page	2293
File Format	PDF
ISSN	19492553
e-ISSN	19492553
Journal	Oncotarget
Issue Number	8
Volume Number	5
Language	English
Publisher	Impact Journals LLC
Publisher Date	2014-04-01
Access Restriction	Open
Rights Holder	Impact Journals LLC
Subject Keyword	Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Oncology

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