NDLI: Effects of Postmortem Interval on Mouse Ovary Oocyte Survival and Maturation

Content Provider	PubMed Central
Author	Zhang, Guang-li Ma, Jun-yu Sun, Quan Hu, Meng-wen Yang, Xiu-yan Gao, Si-hua Jiang, Guang-jian
Editor	Sun, Qing-yuan
Copyright Year	2014
Abstract	To study the time- and temperature-dependent survival of ovarian oocytes collected from postmortem carcass, ICR mice were killed and placed for different periods (0, 1, 2, 4, 6, 8 and 10 h) at different temperatures (25°C, 4°C and 37°C). After preservation, oocyte morphology, germinal vesicle (GV) oocyte number, oocyte meiotic maturation percentage, mitochondrial distribution and intracellular glutathione (GSH) level were evaluated. The results showed no surviving oocytes could be collected by 2h, 6h, and 12 h after carcass preservation at 37°C, 25°C and 4°C, respectively. The number of collected GV oocytes in the ovary deceased as the preservation time lasted at the same temperature. Meanwhile at the same point in time, the ratio of germinal vesicle breakdown (GVBD) and the first polar body emission (PBE) gradually reduced as preservation temperature increased. In addition, the percentage of abnormal mitochondrial distribution in the preserved oocytes was obviously higher than that in the control oocytes, while GSH level was not altered in collected oocytes. Unexpectedly, neither chromosome arrangement nor spindle organization was affected as long as the oocytes from preserved carcasses could complete maturation. These data are helpful for proper use of ovary oocytes from postmortem carcass of valuable individuals.
Related Links	http://dx.doi.org/10.1371/journal.pone.0098384
Starting Page	98384
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	5
Volume Number	9
Language	English
Publisher	Public Library of Science
Publisher Date	2014-05-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Effects of Upregulation of Hsp27 Expression on Oocyte Development and Maturation Derived from Polycystic Ovary Syndrome

Multiple Requirements of PLK1 during Mouse Oocyte Maturation

Effects of 5α-Dihydrotestosterone and 17β-Estradiol on the Mouse Ovarian Follicle Development and Oocyte Maturation

TBP Dynamics during Mouse Oocyte Meiotic Maturation and Early Embryo Development

ING3 Is Essential for Asymmetric Cell Division during Mouse Oocyte Maturation

Symmetry breaking and polarity establishment during mouse oocyte maturation

Probe-Level Analysis of Expression Microarrays Characterizes Isoform-Specific Degradation during Mouse Oocyte Maturation

The Src Homology 2 Domain-Containing Adapter Protein B (SHB) Regulates Mouse Oocyte Maturation

Individual Case Analysis of Postmortem Interval Time on Brain Tissue Preservation

Effects of Postmortem Interval on Mouse Ovary Oocyte Survival and Maturation

Similar Documents

Effects of Upregulation of Hsp27 Expression on Oocyte Development and Maturation Derived from Polycystic Ovary Syndrome

Multiple Requirements of PLK1 during Mouse Oocyte Maturation

Effects of 5α-Dihydrotestosterone and 17β-Estradiol on the Mouse Ovarian Follicle Development and Oocyte Maturation

TBP Dynamics during Mouse Oocyte Meiotic Maturation and Early Embryo Development

ING3 Is Essential for Asymmetric Cell Division during Mouse Oocyte Maturation

Symmetry breaking and polarity establishment during mouse oocyte maturation

Probe-Level Analysis of Expression Microarrays Characterizes Isoform-Specific Degradation during Mouse Oocyte Maturation

The Src Homology 2 Domain-Containing Adapter Protein B (SHB) Regulates Mouse Oocyte Maturation

Individual Case Analysis of Postmortem Interval Time on Brain Tissue Preservation

Effects of Postmortem Interval on Mouse Ovary Oocyte Survival and Maturation