NDLI: Biofabricated Silver Nanoparticles Act as a Strong Fungicide against Bipolaris sorokiniana Causing Spot Blotch Disease in Wheat

Content Provider	PubMed Central
Author	Mishra, Sandhya Singh, Braj Raj Singh, Akanksha Keswani, Chetan Naqvi, Alim H. Singh, H. B.
Editor	Yu, Jae-hyuk
Copyright Year	2014
Abstract	The present study is focused on the extracellular synthesis of silver nanoparticles (AgNPs) using culture supernatant of an agriculturally important bacterium, Serratia sp. BHU-S4 and demonstrates its effective application for the management of spot blotch disease in wheat. The biosynthesis of AgNPs by Serratia sp. BHU-S4 (denoted as bsAgNPs) was monitored by UV–visible spectrum that showed the surface plasmon resonance (SPR) peak at 410 nm, an important characteristic of AgNPs. Furthermore, the structural, morphological, elemental, functional and thermal characterization of bsAgNPs was carried out using the X-ray diffraction (XRD), electron and atomic microscopies, energy dispersive X-ray (EDAX) spectrometer, FTIR spectroscopy and thermogravimetric analyzer (TGA), respectively. The bsAgNPs were spherical in shape with size range of ∼10 to 20 nm. The XRD and EDAX analysis confirmed successful biosynthesis and crystalline nature of AgNPs. The bsAgNPs exhibited strong antifungal activity against Bipolaris sorokiniana, the spot blotch pathogen of wheat. Interestingly, 2, 4 and 10 µg/ml concentrations of bsAgNPs accounted for complete inhibition of conidial germination, whereas in the absence of bsAgNPs, conidial germination was 100%. A detached leaf bioassay revealed prominent conidial germination on wheat leaves infected with B. sorokiniana conidial suspension alone, while the germination of conidia was totally inhibited when the leaves were treated with bsAgNPs. The results were further authenticated under green house conditions, where application of bsAgNPs significantly reduced B. sorokiniana infection in wheat plants. Histochemical staining revealed a significant role of bsAgNPs treatment in inducing lignin deposition in vascular bundles. In summary, our findings represent the efficient application of bsAgNPs in plant disease management, indicating the exciting possibilities of nanofungicide employing agriculturally important bacteria.
Related Links	http://dx.doi.org/10.1371/journal.pone.0097881
Starting Page	97881
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	5
Volume Number	9
Language	English
Publisher	Public Library of Science
Publisher Date	2014-05-19
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Resistance to spot blotch (Bipolaris sorokiniana) disease in bread wheat under changing climate scenario

Screening of wheat germplasm against spot blotch (Bipolaris sorokiniana)

Managing spot blotch disease in wheat: Conventional to molecular aspects

STUDIES ON SPOT BLOTCH [BIPOLARIS SOROKINIANA (SACC.) SHOEM.] OF WHEAT AND ITS MANAGEMENT

Identification and Characterization of Spot Blotch Bipolaris sorokiniana Resistance in Wheat Triticum aestivum L

Understanding the interaction between Bipolaris sorokiniana and wheat during disease development at molecular level

Developing serodiagnostic assay for the detection of Bipolaris sorokiniana causing spot blotch in wheat

A model for forecasting spot blotch disease in wheat

Genetics Of Resistance To Spot Blotch (Bipolaris Sorokiniana Sacc. In Borok, Shoem.) In Wheat (Triticum Aestivum L.Em. Thell.)

Biofabricated Silver Nanoparticles Act as a Strong Fungicide against Bipolaris sorokiniana Causing Spot Blotch Disease in Wheat

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