NDLI: Systemic Delivery of siRNA Down Regulates Brain Prion Protein and Ameliorates Neuropathology in Prion Disorder

Content Provider	PubMed Central
Author	Lehmann, Sylvain Relano-gines, Aroa Resina, Sarah Elsa, Brillaud Casanova, Danielle Vincent, Charles Claire, Hamela Sophie, Poupeau Laffont, Mathieu Gabelle, Audrey Delaby, Constance Belondrade, Maxime Arnaud, Jacques-damien Alvarez, Maria-teresa Maurel, Jean-claude Maurel, Patrick Crozet, Carole
Editor	Chiesa, Roberto
Copyright Year	2014
Abstract	One of the main challenges for neurodegenerative disorders that are principally incurable is the development of new therapeutic strategies, which raises important medical, scientific and societal issues. Creutzfeldt-Jakob diseases are rare neurodegenerative fatal disorders which today remain incurable. The objective of this study was to evaluate the efficacy of the down-regulation of the prion protein (PrP) expression using siRNA delivered by, a water-in-oil microemulsion, as a therapeutic candidate in a preclinical study. After 12 days rectal mucosa administration of Aonys/PrP-siRNA in mice, we observed a decrease of about 28% of the brain PrPC level. The effect of Aonys/PrP-siRNA was then evaluated on prion infected mice. Several mice presented a delay in the incubation and survival time compared to the control groups and a significant impact was observed on astrocyte reaction and neuronal survival in the PrP-siRNA treated groups. These results suggest that a new therapeutic scheme based an innovative delivery system of PrP-siRNA can be envisioned in prion disorders.
Related Links	http://dx.doi.org/10.1371/journal.pone.0088797
Starting Page	88797
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	2
Volume Number	9
Language	English
Publisher	Public Library of Science
Publisher Date	2014-02-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Prion Protein Expression Regulates Embryonic Stem Cell Pluripotency and Differentiation

Prion Protein Accumulation in Lipid Rafts of Mouse Aging Brain

Reducing GBA2 Activity Ameliorates Neuropathology in Niemann-Pick Type C Mice

Prion Protein in Milk

The Cellular Prion Protein Negatively Regulates Phagocytosis and Cytokine Expression in Murine Bone Marrow-Derived Macrophages

Novel siRNA Delivery System to Target Podocytes In Vivo

Effective Delivery of Endogenous Antioxidants Ameliorates Diabetic Nephropathy

eEF-2 Phosphorylation Down-Regulates P-Glycoprotein Over-Expression in Rat Brain Microvessel Endothelial Cells

Immunopurification of Pathological Prion Protein Aggregates

Systemic Delivery of siRNA Down Regulates Brain Prion Protein and Ameliorates Neuropathology in Prion Disorder

Similar Documents

Prion Protein Expression Regulates Embryonic Stem Cell Pluripotency and Differentiation

Prion Protein Accumulation in Lipid Rafts of Mouse Aging Brain

Reducing GBA2 Activity Ameliorates Neuropathology in Niemann-Pick Type C Mice

Prion Protein in Milk

The Cellular Prion Protein Negatively Regulates Phagocytosis and Cytokine Expression in Murine Bone Marrow-Derived Macrophages

Novel siRNA Delivery System to Target Podocytes In Vivo

Effective Delivery of Endogenous Antioxidants Ameliorates Diabetic Nephropathy

eEF-2 Phosphorylation Down-Regulates P-Glycoprotein Over-Expression in Rat Brain Microvessel Endothelial Cells

Immunopurification of Pathological Prion Protein Aggregates

Systemic Delivery of siRNA Down Regulates Brain Prion Protein and Ameliorates Neuropathology in Prion Disorder