NDLI: Homologous electron transport components fail to increase fatty acid hydroxylation in transgenicArabidopsis thaliana

Content Provider	PubMed Central
Author	Wayne, Laura L. Browse, John
Copyright Year	2013
Abstract	Ricinoleic acid, a hydroxylated fatty acid (HFA) present in castor (Ricinus communis) seeds, is an important industrial commodity used in products ranging from inks and paints to polymers and fuels. However, due to the deadly toxin ricin and allergens also present in castor, it would be advantageous to produce ricinoleic acid in a different agricultural crop. Unfortunately, repeated efforts at heterologous expression of the castor fatty acid hydroxylase (RcFAH12) in the model plantArabidopsis thaliana have produced only 17-19% HFA in the seed triacylglycerols (TAG), whereas castor seeds accumulate up to 90% ricinoleic acid in the endosperm TAG. RcFAH12 requires an electron supply from NADH:cytochrome b5 reductase (CBR1) and cytochrome b5 (Cb5) to synthesize ricinoleic acid. Previously, our laboratory found a mutation in theArabidopsis CBR1 gene,cbr1-1, that caused an 85% decrease in HFA levels in the RcFAH12Arabidopsis line. These results raise the possibility that electron supply to the heterologous RcFAH12 may limit the production of HFA. Therefore, we hypothesized that by heterologously expressing RcCb5, the reductant supply to RcFAH12 would be improved and lead to increased HFA accumulation inArabidopsis seeds. Contrary to this proposal, heterologous expression of the top three RcCb5 candidates did not increase HFA accumulation. Furthermore, coexpression of RcCBR1 and RcCb5 in RcFAH12 Arabidopsis also did not increase in HFA levels compared to the parental lines. These results demonstrate that theArabidopsis electron transfer system is supplying sufficient reductant to RcFAH12 and that there must be other bottlenecks limiting the accumulation of HFA.
Related Links	http://dx.doi.org/10.12688/f1000research.2-203.v1
Starting Page	203
File Format	PDF
ISSN	20461402
e-ISSN	20461402
Journal	F1000Research
Volume Number	2
Language	English
Publisher	F1000Research
Publisher Date	2013-10-01
Access Restriction	Open
Rights Holder	F1000Research
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Medicine Biochemistry, Genetics and Molecular Biology Pharmacology, Toxicology and Pharmaceutics

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