NDLI: A mutation in negative regulator of basal resistanceWRKY17 ofArabidopsis increases susceptibility toAgrobacterium-mediated genetic transformation

Content Provider	PubMed Central
Author	Lacroix, Benoît Citovsky, Vitaly
Copyright Year	2013
Abstract	Agrobacterium is a phytopathogenic bacterium that induces crown gall disease in many plant species by transferring and integrating a segment of its own DNA (T-DNA) into its host genome. WhereasAgrobacterium usually does not trigger an extensive defense response in its host plants, it induces the expression of several defense-related genes and activates plant stress reactions. In the complex interplay betweenAgrobacterium and its host plant,Agrobacterium has evolved to take advantage of these plant defense pathways for its own purpose of advancement of the infection process. For example,Agrobacterium utilizes the host stress response transcriptional regulator VIP1 to facilitate nuclear import and proteasomal uncoating of its T-DNA during genetic transformation of the host cell. InArabidopsis, theVIP1 gene expression is repressed by WRKY17, a negative regulator of basal resistance toPseudomonas. Thus, we examined whether WRKY17 is also involved in plant susceptibility to genetic transformation byAgrobacterium. Using reverse genetics, we showed that awrky17 mutant displays higher expression of theVIP1 gene in roots, but not in shoots. In a root infection assay, thewrky17 mutant plants were hyper-susceptible toAgrobacterium compared to wild type plants. WRKY17, therefore, may act as a positive regulator ofArabidopsis resistance toAgrobacterium. This notion is important for understanding the complex regulation ofAgrobacterium-mediated genetic transformation; thus, although this paper reports a relatively small set of data that we do not plan to pursue further in our lab, we believe it might be useful for the broad community of plant pathologists and plant biotechnologists.
Related Links	http://dx.doi.org/10.12688/f1000research.2-33.v1
Starting Page	33
File Format	PDF
ISSN	20461402
e-ISSN	20461402
Journal	F1000Research
Volume Number	2
Language	English
Publisher	F1000Research
Publisher Date	2013-02-01
Access Restriction	Open
Rights Holder	F1000Research
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Medicine Biochemistry, Genetics and Molecular Biology Pharmacology, Toxicology and Pharmaceutics

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