NDLI: Implementation of Microfluidic Sandwich ELISA for Superior Detection of Plant Pathogens

Content Provider	PubMed Central
Author	Thaitrong, Numrin Charlermroj, Ratthaphol Himananto, Orawan Seepiban, Channarong Karoonuthaisiri, Nitsara
Editor	Willson, Richard C.
Copyright Year	2013
Abstract	Rapid and economical screening of plant pathogens is a high-priority need in the seed industry. Crop quality control and disease surveillance demand early and accurate detection in addition to robustness, scalability, and cost efficiency typically required for selective breeding and certification programs. Compared to conventional bench-top detection techniques routinely employed, a microfluidic-based approach offers unique benefits to address these needs simultaneously. To our knowledge, this work reports the first attempt to perform microfluidic sandwich ELISA for Acidovorax citrulli (Ac), watermelon silver mottle virus (WSMoV), and melon yellow spot virus (MYSV) screening. The immunoassay occurs on the surface of a reaction chamber represented by a microfluidic channel. The capillary force within the microchannel draws a reagent into the reaction chamber as well as facilitates assay incubation. Because the underlying pad automatically absorbs excess fluid, the only operation required is sequential loading of buffers/reagents. Buffer selection, antibody concentrations, and sample loading scheme were optimized for each pathogen. Assay optimization reveals that the 20-folds lower sample volume demanded by the microchannel structure outweighs the 2- to 4-folds higher antibody concentrations required, resulting in overall 5–10 folds of reagent savings. In addition to cutting the assay time by more than 50%, the new platform offers 65% cost savings from less reagent consumption and labor cost. Our study also shows 12.5-, 2-, and 4-fold improvement in assay sensitivity for Ac, WSMoV, and MYSV, respectively. Practical feasibility is demonstrated using 19 real plant samples. Given a standard 96-well plate format, the developed assay is compatible with commercial fluorescent plate readers and readily amendable to robotic liquid handling systems for completely hand-free assay automation.
Related Links	http://dx.doi.org/10.1371/journal.pone.0083231
Starting Page	83231
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	12
Volume Number	8
Language	English
Publisher	Public Library of Science
Publisher Date	2013-12-23
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Development of a double-antibody sandwich ELISA for rapid detection of Bacillus Cereus in food

Monoclonal Antibody-Based Sandwich ELISA for the Detection of Staphylococcal Enterotoxin A

Detection of β-Lactamase Residues in Milk by Sandwich ELISA

Development of Monoclonal Antibody-Based Sandwich ELISA for Detection of Dextran

A Sandwich ELISA for the Detection of Wnt5a

Detection of Coproantigens by Sandwich ELISA in Rabbits Experimentally Infected with Fasciola gigantica

Sandwich ELISA for quantitative detection of human collagen prolyl 4-hydroxylase

Evaluation of Monoclonal Antibody-Based Sandwich Direct ELISA (MSD-ELISA) for Antigen Detection of Foot-and-Mouth Disease Virus Using Clinical Samples

Correction: Evaluation of Monoclonal Antibody-Based Sandwich Direct ELISA (MSD-ELISA)for Antigen Detection of Foot-and-Mouth Disease Virus Using Clinical Samples

Implementation of Microfluidic Sandwich ELISA for Superior Detection of Plant Pathogens

Similar Documents

Development of a double-antibody sandwich ELISA for rapid detection of Bacillus Cereus in food

Monoclonal Antibody-Based Sandwich ELISA for the Detection of Staphylococcal Enterotoxin A

Detection of β-Lactamase Residues in Milk by Sandwich ELISA

Development of Monoclonal Antibody-Based Sandwich ELISA for Detection of Dextran

A Sandwich ELISA for the Detection of Wnt5a

Detection of Coproantigens by Sandwich ELISA in Rabbits Experimentally Infected with Fasciola gigantica

Sandwich ELISA for quantitative detection of human collagen prolyl 4-hydroxylase

Evaluation of Monoclonal Antibody-Based Sandwich Direct ELISA (MSD-ELISA) for Antigen Detection of Foot-and-Mouth Disease Virus Using Clinical Samples

Correction: Evaluation of Monoclonal Antibody-Based Sandwich Direct ELISA (MSD-ELISA)for Antigen Detection of Foot-and-Mouth Disease Virus Using Clinical Samples

Implementation of Microfluidic Sandwich ELISA for Superior Detection of Plant Pathogens