NDLI: Absence of Synaptic Regulation by Phosducin in Retinal Slices

Content Provider	PubMed Central
Author	Long, James H. Arshavsky, Vadim Y. Burns, Marie E.
Editor	Taylor, William Rowland
Copyright Year	2013
Abstract	Phosducin is an abundant photoreceptor protein that binds G-protein βγ subunits and plays a role in modulating synaptic transmission at photoreceptor synapses under both dark-adapted and light-adapted conditions in vivo. To examine the role of phosducin at the rod-to-rod bipolar cell (RBC) synapse, we used whole-cell voltage clamp recordings to measure the light-evoked currents from both wild-type (WT) and phosducin knockout (Pd−/− ) RBCs, in dark- and light-adapted retinal slices. Pd−/− RBCs showed smaller dim flash responses and steeper intensity-response relationships than WT RBCs, consistent with the smaller rod responses being selectively filtered out by the non-linear threshold at the rod-to-rod bipolar synapse. In addition, Pd−/− RBCs showed a marked delay in the onset of the light-evoked currents, similar to that of a WT response to an effectively dimmer flash. Comparison of the changes in flash sensitivity in the presence of steady adapting light revealed that Pd−/− RBCs desensitized less than WT RBCs to the same intensity. These results are quantitatively consistent with the smaller single photon responses of Pd−/− rods, owing to the known reduction in rod G-protein expression levels in this line. The absence of an additional synaptic phenotype in these experiments suggests that the function of phosducin at the photoreceptor synapse is abolished by the conditions of retinal slice recordings.
Related Links	http://dx.doi.org/10.1371/journal.pone.0083970
Starting Page	83970
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	12
Volume Number	8
Language	English
Publisher	Public Library of Science
Publisher Date	2013-12-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Retinal Cone Photoreceptors Require Phosducin-Like Protein 1 for G Protein Complex Assembly and Signaling

Reduced Retinal Function in the Absence of Nav1.6

Regulation of Synaptic Vesicle Docking by Different Classes of Macromolecules in Active Zone Material

Dopamine D1 Receptors Regulate the Light Dependent Development of Retinal Synaptic Responses

Evolution of the Aging Brain Transcriptome and Synaptic Regulation

MicroRNA Regulation of the Synaptic Plasticity-Related Gene Arc

Correction: Dopamine D1 Receptors Regulate the Light Dependent Development of Retinal Synaptic Responses

Lesions in the Posterior Visual Pathway Promote Trans-Synaptic Degeneration of Retinal Ganglion Cells

The role of retinal waves and synaptic normalization in retinogeniculate development.

Absence of Synaptic Regulation by Phosducin in Retinal Slices

Similar Documents

Retinal Cone Photoreceptors Require Phosducin-Like Protein 1 for G Protein Complex Assembly and Signaling

Reduced Retinal Function in the Absence of Nav1.6

Regulation of Synaptic Vesicle Docking by Different Classes of Macromolecules in Active Zone Material

Dopamine D1 Receptors Regulate the Light Dependent Development of Retinal Synaptic Responses

Evolution of the Aging Brain Transcriptome and Synaptic Regulation

MicroRNA Regulation of the Synaptic Plasticity-Related Gene Arc

Correction: Dopamine D1 Receptors Regulate the Light Dependent Development of Retinal Synaptic Responses

Lesions in the Posterior Visual Pathway Promote Trans-Synaptic Degeneration of Retinal Ganglion Cells

The role of retinal waves and synaptic normalization in retinogeniculate development.

Absence of Synaptic Regulation by Phosducin in Retinal Slices