NDLI: Structural insights into the Ca2+ and PI(4,5)P2 binding modes of the C2 domains of rabphilin 3A and synaptotagmin 1

Content Provider	PubMed Central
Author	Guillén, Jaime Cristina, Ferrer-orta Buxaderas, Mònica Dolores, Pérez-sánchez Marta, Guerrero-valero Ginés, Luengo-gil Pous, Joan Guerra, Pablo Gómez-fernández, Juan C. Verdaguer, Nuria Corbalán-garcía, Senena
Abstract	Vesicle fusion is an important event in neuronal transmission and endocrine cell secretion. A myriad of proteins containing double C2 domains are involved in this complex process; however, how Ca2+ and the different types of membrane lipids regulate their function is still not well understood. In this work, we provide structural insights to explain the ability of different C2 domains to interact with Ca2+ and PI(4,5)P2 and demonstrate the existence of a specific PI(4,5)P2-binding motif that provides these domains with specific properties to interact with the membrane and initiate vesicle fusion. We also demonstrate a unique molecular mechanism conferring their specificity for the different phosphoinositides, which resides in additional amino acidic residues surrounding the key interacting lysines.
Related Links	http://dx.doi.org/10.1073/pnas.1316179110
Ending Page	20508
Page Count	6
Starting Page	20503
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	51
Volume Number	110
Language	English
Publisher	National Academy of Sciences
Publisher Date	2013-12-17
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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