NDLI: Intramolecular clasp of the cellulosomal Ruminococcus flavefaciens ScaA dockerin module confers structural stability ☆

Content Provider	PubMed Central
Author	Michal, Slutzki Jobby, Maroor K. Seth, Chitayat Karpol, Alon Dassa, Bareket Barak, Yoav Raphael, Lamed Smith, Steven P. Bayer, Edward A.
Copyright Year	2013
Abstract	The cellulosome is a large extracellular multi-enzyme complex that facilitates the efficient hydrolysis and degradation of crystalline cellulosic substrates. During the course of our studies on the cellulosome of the rumen bacterium Ruminococcus flavefaciens, we focused on the critical ScaA dockerin (ScaADoc), the unique dockerin that incorporates the primary enzyme-integrating ScaA scaffoldin into the cohesin-bearing ScaB adaptor scaffoldin. In the absence of a high-resolution structure of the ScaADoc module, we generated a computational model, and, upon its analysis, we were surprised to discover a putative stacking interaction between an N-terminal Trp and a C-terminal Pro, which we termed intramolecular clasp. In order to verify the existence of such an interaction, these residues were mutated to alanine. Circular dichroism spectroscopy, intrinsic tryptophan and ANS fluorescence, and NMR spectroscopy indicated that mutation of these residues has a destabilizing effect on the functional integrity of the Ca2+-bound form of ScaADoc. Analysis of recently determined dockerin structures from other species revealed the presence of other well-defined intramolecular clasps, which consist of different types of interactions between selected residues at the dockerin termini. We propose that this thematic interaction may represent a major distinctive structural feature of the dockerin module.
Related Links	http://dx.doi.org/10.1016/j.fob.2013.09.006
Ending Page	405
Page Count	8
Starting Page	398
File Format	PDF
ISSN	22115463
e-ISSN	22115463
Journal	FEBS Open Bio
Volume Number	3
Language	English
Publisher	Elsevier
Publisher Date	2013-01-01
Access Restriction	Open
Rights Holder	Elsevier
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology

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Cellulosomal Scaffoldin-Like Proteins from Ruminococcus flavefaciens

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Intramolecular clasp of the cellulosomal Ruminococcus flavefaciens ScaA dockerin module confers structural stability ☆

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