NDLI: Evidence for Mitotic Recombination within the Basidia of a Hybrid Cross of Cryptococcus neoformans

Content Provider	PubMed Central
Author	Vogan, Aaron A. Jordan, Khankhet Jianping, Xu
Editor	Nielsen, Kirsten
Copyright Year	2013
Abstract	In the majority of diploid eukaryotes, each meiotic process generates four haploid gametes with each containing a single recombinant nucleus. In some species and/or some meiotic processes, aneuploid or diploid gametes can also be generated due to chromosomal non-disjunction and/or the co-packaging of two of the four haploid nuclei into the same gamete. Here we show that another process is involved in generating genotypes of sexual progeny from a hybrid cross between two divergent lineages of the human fungal pathogen Cryptococcus neoformans. Through micro-dissection of 1358 basidiospores from 194 basidia and genotyping using 33 co-dominant genetic markers, the genotypes of all 230 germinated basidiospores from 94 basidia were obtained. The minimum haploid genotypes required to constitute the observed genotypes from each basidium were then inferred. Our results demonstrated that more than four haploid nuclear genotypes are required to explain the observed genotypes of basidiospores in seven of the 94 basidia. Our results suggest that mitotic recombination within basidia must be involved to produce the observed genotypes in these seven basidia. The mitotic recombination likely includes both chromosomal loss and crossing over. This novel recombination process could play an important role in generating the genotypic and phenotypic diversities of this important human pathogen.
Related Links	http://dx.doi.org/10.1371/journal.pone.0062790
Starting Page	62790
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	5
Volume Number	8
Language	English
Publisher	Public Library of Science
Publisher Date	2013-05-14
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Light microscopy of basidia, basidiospores, and nuclei in spores and hyphae of Filobasidiella neoformans (Cryptococcus neoformans).

Evidence of Sexual Recombination among Cryptococcus neoformans Serotype A Isolates in Sub-Saharan Africa†

Genetic Analyses of a Hybrid Cross Between Serotypes A and D Strains of the Human Pathogenic Fungus Cryptococcus neoformans

Evidence for Mitotic Recombination in the Cellular Slime Mold Dictyostelium discoideum

Epidemiological Evidence for Dormant Cryptococcus neoformans Infection

Cryptococcus neoformans var. grubii: Separate Varietal Status for Cryptococcus neoformans Serotype A Isolates

Development of a Singleplex PCR Assay for Rapid Identification and Differentiation of Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, Cryptococcus gattii, and Hybrids

Evidence that spontaneous mitotic recombination occurs at the two-strand stage.

Improved diagnostic medium for separation of Cryptococcus neoformans var. neoformans (serotypes A and D) and Cryptococcus neoformans var. gattii (serotypes B and C).

Evidence for Mitotic Recombination within the Basidia of a Hybrid Cross of Cryptococcus neoformans

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Light microscopy of basidia, basidiospores, and nuclei in spores and hyphae of Filobasidiella neoformans (Cryptococcus neoformans).

Evidence of Sexual Recombination among Cryptococcus neoformans Serotype A Isolates in Sub-Saharan Africa†

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Evidence for Mitotic Recombination within the Basidia of a Hybrid Cross of Cryptococcus neoformans