NDLI: Phylogenetic and Evolutionary Analysis of Chinese Leishmania Isolates Based on Multilocus Sequence Typing

Content Provider	PubMed Central
Author	Zhang, Chun-ying Lu, Xiao-jun Du, Xiao-qing Jian, Jun Shu, Ling Ying, Ma
Editor	Zilberstein, Dan
Copyright Year	2013
Abstract	Leishmaniasis is a debilitating infectious disease that has a variety of clinical forms. In China, visceral leishmaniasis (VL) is the most common symptom, and L. donovani and/or L. infantum are the likely pathogens. In this study, multilocus sequence typing (MLST) of five enzyme-coding genes (fh, g6pdh, icd, mpi, pgd) and two conserved genes (hsp70, lack) was used to investigate the phylogenetic relationships of Chinese Leishmania strains. Concatenated alignment of the nucleotide sequences of the seven genes was analyzed and phylogenetic trees were constructed using neighbor-joining and maximum parsimony models. A set of additional sequences from 25 strains (24 strains belong to the L. donovani complex and one strain belongs to L. gerbilli) were retrieved from GenBank to infer the molecular evolutionary history of Leishmania from China and other endemic areas worldwide. Phylogenetic analyses consolidated Chinese Leishmania into four groups: (i) one clade A population comprised 13 isolates from different foci in China, which were pathogenic to humans and canines. This population was subdivided into two subclades, clade A1 and clade A2, which comprised sister organisms to the remaining members of the worldwide L. donovani complex; (ii) a population in clade B consisted of one reference strain of L. turanica and five Chinese strains from Xinjiang; (iii) clade C (SELF-7 and EJNI-154) formed a population that was closely related to clade B, and both isolates were identified as L. gerbilli; and (iv) the final group, clade D, included Sauroleishmania (LIZRD and KXG-E) and was distinct from the other strains. We hypothesize that the phylogeny of Chinese Leishmania is associated with the geographical origins rather than with the clinical forms (VL or CL) of leishmaniasis. To conclude, this study provides further molecular information on Chinese Leishmania isolates and the Chinese isolates appear to have a more complex evolutionary history than previously thought.
Related Links	http://dx.doi.org/10.1371/journal.pone.0063124
Starting Page	63124
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	4
Volume Number	8
Language	English
Publisher	Public Library of Science
Publisher Date	2013-04-30
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Prevalence and genetic diversity of clinical Vibrio parahaemolyticus isolates from China, revealed by multilocus sequence typing scheme

Analysis of Multilocus Sequence Typing and Virulence Characterization of Listeria monocytogenes Isolates from Chinese Retail Ready-to-Eat Food

Multilocus Sequence Typing and Phylogenetic Analysis of Propionibacterium acnes

OUTER MEMBRANE PROTEIN PROFILE ANALYSIS AND MULTILOCUS SEQUENCE TYPING OF PASTEURELLA MULTOCIDA ISOLATES OF BUFFALO ORIGIN FROM GUJARAT STATE

OUTER MEMBRANE PROTEIN PROFILE ANALYSIS AND MULTILOCUS SEQUENCE TYPING OF PASTEVREllA MULTOCIDA ISOLATES OF BUFFALO ORIGIN FROM GUJARAT STATE

Multilocus microsatellite typing of Leishmania infantum isolates in monitored Leishmania/HIV coinfected patients

Assignment of Staphylococcus Isolates to Groups by spa Typing, SmaI Macrorestriction Analysis, and Multilocus Sequence Typing

Analysis of Multilocus Sequence Typing for Identification of Leptospira Isolates in Brazil ▿

Multilocus sequence typing of clinical and colonizing isolates of Acinetobacter baumannii and comparison with the world isolates

Phylogenetic and Evolutionary Analysis of Chinese Leishmania Isolates Based on Multilocus Sequence Typing

Similar Documents

Prevalence and genetic diversity of clinical Vibrio parahaemolyticus isolates from China, revealed by multilocus sequence typing scheme

Analysis of Multilocus Sequence Typing and Virulence Characterization of Listeria monocytogenes Isolates from Chinese Retail Ready-to-Eat Food

Multilocus Sequence Typing and Phylogenetic Analysis of Propionibacterium acnes

OUTER MEMBRANE PROTEIN PROFILE ANALYSIS AND MULTILOCUS SEQUENCE TYPING OF PASTEURELLA MULTOCIDA ISOLATES OF BUFFALO ORIGIN FROM GUJARAT STATE

OUTER MEMBRANE PROTEIN PROFILE ANALYSIS AND MULTILOCUS SEQUENCE TYPING OF PASTEVREllA MULTOCIDA ISOLATES OF BUFFALO ORIGIN FROM GUJARAT STATE

Multilocus microsatellite typing of Leishmania infantum isolates in monitored Leishmania/HIV coinfected patients

Assignment of Staphylococcus Isolates to Groups by spa Typing, SmaI Macrorestriction Analysis, and Multilocus Sequence Typing

Analysis of Multilocus Sequence Typing for Identification of Leptospira Isolates in Brazil ▿

Multilocus sequence typing of clinical and colonizing isolates of Acinetobacter baumannii and comparison with the world isolates

Phylogenetic and Evolutionary Analysis of Chinese Leishmania Isolates Based on Multilocus Sequence Typing