NDLI: E2F mediates dihydrofolate reductase promoter activation and multiprotein complex formation in human cytomegalovirus infection.

Please wait, while we are loading the content...

Year: 2016, Volume: 36

Year: 2015, Volume: 36

Year: 2015, Volume: 35

Year: 2014, Volume: 35

Year: 2014, Volume: 34

Year: 2013, Volume: 33

Year: 2012, Volume: 32

Year: 2011, Volume: 31

Year: 2010, Volume: 30

Year: 2009, Volume: 29

Year: 2008, Volume: 28

Year: 2007, Volume: 27

Year: 2006, Volume: 26

Year: 2005, Volume: 25

Year: 2004, Volume: 24

Year: 2003, Volume: 23

Year: 2002, Volume: 23

Year: 2002, Volume: 22

Year: 2001, Volume: 21

Year: 2000, Volume: 20

Year: 1999, Volume: 19

Year: 1998, Volume: 18

Year: 1997, Volume: 17

Year: 1996, Volume: 16

Year: 1995, Volume: 15

Year: 1994, Volume: 14

Year: 1993, Volume: 13

Year: 1992, Volume: 12

Year: 1992, Volume: 12, Issue: 12

Year: 1992, Volume: 12, Issue: 11

Year: 1992, Volume: 12, Issue: 10

Cloning of GT box-binding proteins: a novel Sp1 multigene family regulating T-cell receptor gene expression.

Unusual aspects of in vitro RNA processing in the 3' regions of the GAL1, GAL7, and GAL10 genes in Saccharomyces cerevisiae.

Regulated expression of a mammalian nonsense suppressor tRNA gene in vivo and in vitro using the lac operator/repressor system.

The spliceosome assembly pathway in mammalian extracts.

Different chromatin structures along the spacers flanking active and inactive Xenopus rRNA genes.

Characterization of Spi-B, a transcription factor related to the putative oncoprotein Spi-1/PU.1.

The ARS consensus sequence is required for chromosomal origin function in Saccharomyces cerevisiae.

RPC53 encodes a subunit of Saccharomyces cerevisiae RNA polymerase C (III) whose inactivation leads to a predominantly G1 arrest.

Identification of a 60-kilodalton Rb-binding protein, RBP60, that allows the Rb-E2F complex to bind DNA.

The MRF4 activation domain is required to induce muscle-specific gene expression.

Differential effects of carboxy-terminal sequence deletions on platelet-derived growth factor receptor signaling activities and interactions with cellular substrates.

Regulation of T-cell lymphokine gene transcription by the accessory molecule CD28.

E2F mediates dihydrofolate reductase promoter activation and multiprotein complex formation in human cytomegalovirus infection.

The plant amino acid mimosine may inhibit initiation at origins of replication in Chinese hamster cells.

A single MEF-2 site is a major positive regulatory element required for transcription of the muscle-specific subunit of the human phosphoglycerate mutase gene in skeletal and cardiac muscle cells.

Promoter-specific trans-activation by the adenovirus E1A12S product involves separate E1A domains.

Histone H2B gene transcription during Xenopus early development requires functional cooperation between proteins bound to the CCAAT and octamer motifs.

Selection of optimal kappa B/Rel DNA-binding motifs: interaction of both subunits of NF-kappa B with DNA is required for transcriptional activation.

Ectopic recombination within homologous immunoglobulin mu gene constant regions in a mouse hybridoma cell line.

RPC82 encodes the highly conserved, third-largest subunit of RNA polymerase C (III) from Saccharomyces cerevisiae.

Ectopic recombination between Ty elements in Saccharomyces cerevisiae is not induced by DNA damage.

Accumulation of U14 small nuclear RNA in Saccharomyces cerevisiae requires box C, box D, and a 5', 3' terminal stem.

A CD44-like endothelial cell transmembrane glycoprotein (GP116) interacts with extracellular matrix and ankyrin.

Mapping of epidermal growth factor-, serum-, and phorbol ester-responsive sequence elements in the c-jun promoter.

Cyclic AMP-dependent protein kinase inhibits the activity of myogenic helix-loop-helix proteins.

Modulation of interferon signaling in human fibroblasts by phorbol esters.

A bipartite suppressor: conjunction of two distinct factor-binding sites is essential for down-regulation in rat epoxide hydrolase gene expression.

Involvement of the SIN4 global transcriptional regulator in the chromatin structure of Saccharomyces cerevisiae.

The posttranslational processing of ras p21 is critical for its stimulation of yeast adenylate cyclase.

Effects of p56lck deficiency on the growth and cytolytic effector function of an interleukin-2-dependent cytotoxic T-cell line.

Examination of the DNA-binding ability of estrogen receptor in whole cells: implications for hormone-independent transactivation and the actions of antiestrogens.

Cutinase in Cryphonectria parasitica, the chestnut blight fungus: suppression of cutinase gene expression in isogenic hypovirulent strains containing double-stranded RNAs.

Purification of splicing factor SF1, a heat-stable protein that functions in the assembly of a presplicing complex.

Mutations in the WSAWSE and cytosolic domains of the erythropoietin receptor affect signal transduction and ligand binding and internalization.

Distinct roles for the two cGATA-1 finger domains.

In vivo transcriptional analysis of the TATA-less promoter of the Drosophila melanogaster vermilion gene.

Multiple elements in the upstream glucokinase promoter contribute to transcription in insulinoma cells.

Sequences in the human c-myc P2 promoter affect the elongation and premature termination of transcripts initiated from the upstream P1 promoter.

The yeast EUG1 gene encodes an endoplasmic reticulum protein that is functionally related to protein disulfide isomerase.

Downregulation of JE and KC genes by glucocorticoids does not prevent the G0----G1 transition in BALB/3T3 cells.

Characterization of the human lipoprotein lipase (LPL) promoter: evidence of two cis-regulatory regions, LP-alpha and LP-beta, of importance for the differentiation-linked induction of the LPL gene during adipogenesis.

Localization of the rap1GAP catalytic domain and sites of phosphorylation by mutational analysis.

Transgenic overexpression of transforming growth factor alpha bypasses the need for c-Ha-ras mutations in mouse skin tumorigenesis.

Interactions among LRF-1, JunB, c-Jun, and c-Fos define a regulatory program in the G1 phase of liver regeneration.

COUP orphan receptors are negative regulators of retinoic acid response pathways.

Mutations in a 19-amino-acid hydrophobic region of the yeast cytochrome c1 presequence prevent sorting to the mitochondrial intermembrane space.

Mutated alpha subunit of the Gq protein induces malignant transformation in NIH 3T3 cells.

Jun is phosphorylated by several protein kinases at the same sites that are modified in serum-stimulated fibroblasts.

Activation of c-Src in cells bearing v-Crk and its suppression by Csk.

Regulation of collagen I gene expression by ras.

Activated lck tyrosine protein kinase stimulates antigen-independent interleukin-2 production in T cells.

Localization of a DNA replication origin and termination zone on chromosome III of Saccharomyces cerevisiae.

Biphasic increase in c-jun mRNA is required for induction of AP-1-mediated gene transcription: differential effects of muscarinic and thrombin receptor activation.

Molecular involvement of the pvt-1 locus in a gamma/delta T-cell leukemia bearing a variant t(8;14)(q24;q11) translocation.

Analysis of the defect in DNA end joining in the murine scid mutation.

Maximal serum stimulation of the c-fos serum response element requires both the serum response factor and a novel binding factor, SRE-binding protein.

A proposed mechanism for promoter-associated DNA rearrangement events at a variant surface glycoprotein gene expression site.

cis-acting elements involved in the alternative translation initiation process of human basic fibroblast growth factor mRNA.

GAL11 protein, an auxiliary transcription activator for genes encoding galactose-metabolizing enzymes in Saccharomyces cerevisiae.

Construction of a Bifunctional mRNA in the Mouse by Using the Internal Ribosomal Entry Site of the Encephalomyocarditis Virus

Year: 1992, Volume: 12, Issue: 9

Year: 1992, Volume: 12, Issue: 8

Year: 1992, Volume: 12, Issue: 7

Year: 1992, Volume: 12, Issue: 6

Year: 1992, Volume: 12, Issue: 5

Year: 1992, Volume: 12, Issue: 4

Year: 1992, Volume: 12, Issue: 3

Year: 1992, Volume: 12, Issue: 2

Year: 1992, Volume: 12, Issue: 1

Year: 1991, Volume: 11

Year: 1990, Volume: 10

Year: 1989, Volume: 9

Year: 1988, Volume: 8

Year: 1987, Volume: 7

Year: 1986, Volume: 6

Year: 1985, Volume: 5

Year: 1984, Volume: 4

Year: 1983, Volume: 3

Year: 1982, Volume: 2

Year: 1981, Volume: 1

E2F mediates dihydrofolate reductase promoter activation and multiprotein complex formation in human cytomegalovirus infection.

Content Provider	PubMed Central
Author	Wade, M. Kowalik, T. F. Mudryj, M. Huang, E. S. Azizkhan, J. C.
Abstract	The adenovirus immediate-early protein E1A activates the adenovirus E2 promoter and several cellular gene promoters through transcription factor E2F. The immediate-early proteins of human cytomegalovirus (HCMV) can complement an E1A-deficient adenovirus mutant and activate the adenovirus E2 promoter. HCMV also has been shown to activate the adenovirus E2 promoter. On the basis of these findings, we have investigated whether HCMV can activate the promoter of the cellular dihydrofolate reductase (DHFR) gene, which requires E2F binding for maximal promoter activity. We show that HCMV activates the DHFR promoter and that products of the HCMV major immediate-early gene region mediate the activation of the promoter specifically through the E2F site. We used gel mobility shift assays to search for potential molecular mechanisms for this activation and found an "infection-specific" multimeric complex that bound to the E2F sites in the DHFR and E2 promoters in extracts from HCMV-infected cells but not in extracts from uninfected cells. Several antibodies against HCMV immediate-early gene products had no effect on this infection-specific complex. Subsequently, the complex was found to contain E2F, cyclin A, p33cdk2, and p107 and to be similar to S-phase-specific complexes that recently have been identified in several cell types. A functional role for the binding of the cyclin A-p33cdk2 complex to cellular gene promoters has yet to be demonstrated; however, HCMV infection causes the induction of both cellular DNA replication and transcription of growth-related genes containing E2F sites in their promoters. The findings described above therefore may relate to both of these effects of HCMV infection. We also provide evidence that some of the molecular events associated with adenovirus infection are different from those associated with HCMV infection.
Starting Page	4364
File Format	PDF
ISSN	10985549
e-ISSN	10985549
Journal	Molecular and Cellular Biology
Issue Number	10
Volume Number	12
Language	English
Publisher Date	1992-10-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in