NDLI: Effects of expression level of DNA repair-related genes involved in the NHEJ pathway on radiation-induced cognitive impairment

Content Provider	PubMed Central
Author	Zhang, Li-yuan Chen, Lie-song Sun, Rui Ji, Sheng-jun Ding, Yan-yan Wu, Jia Tian, Ye
Copyright Year	2012
Abstract	Cranial radiation therapy can induce cognitive decline. Impairments of hippocampal neurogenesis are thought to be a paramountly important mechanism underlying radiation-induced cognitive dysfunction. In the mature nervous system, DNA double-strand breaks (DSBs) are mainly repaired by non-homologous end-joining (NHEJ) pathways. It has been demonstrated that NHEJ deficiencies are associated with impaired neurogenesis. In our study, rats were randomly divided into five groups to be irradiated by single doses of 0 (control), 0 (anesthesia control), 2, 10, and 20 Gy, respectively. The cognitive function of the irradiated rats was measured by open field, Morris water maze and passive avoidance tests. Real-time PCR was also used to detect the expression level of DNA DSB repair-related genes involved in the NHEJ pathway, such as XRCC4, XRCC5and XRCC6, in the hippocampus. The influence of different radiation doses on cognitive function in rats was investigated. From the results of the behavior tests, we found that rats receiving 20 Gy irradiation revealed poorer learning and memory, while no significant loss of learning and memory existed in rats receiving irradiation from 0–10 Gy. The real-time PCR and Western blot results showed no significant difference in the expression level of DNA repair-related genes between the 10 and 20 Gy groups, which may help to explain the behavioral results, i.e. DNA damage caused by 0–10 Gy exposure was appropriately repaired, however, damage induced by 20 Gy exceeded the body's maximum DSB repair ability. Ionizing radiation-induced cognitive impairments depend on the radiation dose, and more directly on the body's own ability to repair DNA DSBs via the NHEJ pathway.
Related Links	http://dx.doi.org/10.1093/jrr/rrs095
Ending Page	242
Page Count	8
Starting Page	235
File Format	PDF
ISSN	04493060
e-ISSN	13499157
Journal	Journal of Radiation Research
Issue Number	2
Volume Number	54
Language	English
Publisher	Oxford University Press
Publisher Date	2013-03-01
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Health, Toxicology and Mutagenesis Radiation Radiology Nuclear Medicine and imaging Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Radiology, Nuclear Medicine and Imaging Health, Toxicology and Mutagenesis Radiation

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Effects of expression level of DNA repair-related genes involved in the NHEJ pathway on radiation-induced cognitive impairment

Effects of expression level of DNA repair-related genes involved in the NHEJ pathway on radiation-induced cognitive impairment (2012)

Effects of expression level of DNA repair-related genes involved in the NHEJ pathway on radiation-induced cognitive impairment (2012)

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