NDLI: Wortmannin efficiently suppresses the recovery from radiation-induced damage in pimonidazole-unlabeled quiescent tumor cell population

Content Provider	PubMed Central
Author	Masunaga, Shin-ichiro Sakurai, Yoshinori Tanaka, Hiroki Suzuki, Minoru Kondo, Natsuko Narabayashi, Masaru Maruhashi, Akira Ono, Koji
Copyright Year	2012
Abstract	Labeling of proliferating (P) cells in mice bearing EL4 tumors was achieved by continuous administration of 5-bromo-2′-deoxyuridine (BrdU). Tumors were irradiated with γ-rays at 1 h after pimonidazole administration followed by caffeine or wortmannin treatment. Twenty-four hours later, assessment of the responses of quiescent (Q) and total (= P + Q) cell populations were based on the frequencies of micronucleation and apoptosis using immunofluorescence staining for BrdU. The response of the pimonidazole-unlabeled tumor cell fractions was assessed by means of apoptosis frequency using immunofluorescence staining for pimonidazole. The pimonidazole-unlabeled cell fraction showed significantly enhanced radio-sensitivity compared with the whole cell fraction more remarkably in Q cells than total cells. However, a significantly greater decrease in radio-sensitivity in the pimonidazole-unlabeled than the whole cell fraction, evaluated using an assay performed 24 hours after irradiation, was more clearly observed in Q cells than total cells. In both the pimonidazole-unlabeled and the whole cell fractions, wortmannin efficiently suppressed the reduction in sensitivity due to delayed assay. Wortmannin combined with γ-ray irradiation is useful for suppressing the recovery from radiation-induced damage especially in the pimonidazole-unlabeled cell fraction within the total and Q tumor cell populations.
Related Links	http://dx.doi.org/10.1093/jrr/rrs094
Ending Page	229
Page Count	9
Starting Page	221
File Format	PDF
ISSN	04493060
e-ISSN	13499157
Journal	Journal of Radiation Research
Issue Number	2
Volume Number	54
Language	English
Publisher	Oxford University Press
Publisher Date	2013-03-01
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Health, Toxicology and Mutagenesis Radiation Radiology Nuclear Medicine and imaging Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Radiology, Nuclear Medicine and Imaging Health, Toxicology and Mutagenesis Radiation

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