NDLI: Mycobacterium-Inducible Nramp in Striped Bass (Morone saxatilis)†

Content Provider	PubMed Central
Author	Burge, Erin J. Gauthier, David T. Ottinger, Christopher A. Veld, Peter A. Van
Copyright Year	2004
Abstract	In mammals, the natural resistance-associated macrophage protein 1 gene, Nramp1, plays a major role in resistance to mycobacterial infections. Chesapeake Bay striped bass (Morone saxatilis) is currently experiencing an epizootic of mycobacteriosis that threatens the health of this ecologically and economically important species. In the present study, we characterized an Nramp gene in this species and obtained evidence that there is induction following Mycobacterium exposure. The striped bass Nramp gene (MsNramp) and a 554-amino-acid sequence contain all the signal features of the Nramp family, including a topology of 12 transmembrane domains (TM), the transport protein-specific binding-protein-dependent transport system inner membrane component signature, three N-linked glycosylation sites between TM 7 and TM 8, sites of casein kinase and protein kinase C phosphorylation in the amino and carboxy termini, and a tyrosine kinase phosphorylation site between TM 6 and TM 7. Phylogenetic analysis most closely grouped MsNramp with other teleost Nramp genes and revealed high sequence similarity with mammalian Nramp2. MsNramp expression was present in all tissues assayed by reverse transcription-PCR. Within 1 day of injection of Mycobacterium marinum, MsNramp expression was highly induced (17-fold higher) in peritoneal exudate (PE) cells compared to the expression in controls. The levels of MsNramp were three- and sixfold higher on days 3 and 15, respectively. Injection of Mycobacterium shottsii resulted in two-, five-, and threefold increases in gene expression in PE cells over the time course. This report is the first report of induction of an Nramp gene by mycobacteria in a poikilothermic vertebrate.
Related Links	http://dx.doi.org/10.1128/iai.72.3.1626-1636.2004
Ending Page	1636
Page Count	11
Starting Page	1626
File Format	PDF
ISSN	00199567
e-ISSN	10985522
Journal	Infection and Immunity
Issue Number	3
Volume Number	72
Language	English
Publisher	American Society for Microbiology
Publisher Date	2004-03-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Immunology Microbiology Parasitology Infectious Diseases Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Parasitology Immunology Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Mycobacterium-inducible Nramp in striped bass (Morone saxatilis).

A unique Mycobacterium species isolated from an epizootic of striped bass (Morone saxatilis).

Genes from the striped bass, morone saxatilis.

Molecular characterization of a rotaviruslike virus isolated from striped bass (Morone saxatilis).

A Unique Mycobacterium Species Isolated from an Epizootic of Striped Bass (Morone saxatilis)

A unique Mycobacterium species isolated from an epizootic of striped bass (Morone saxatilis).

Pasteurellosis in Reared Hybrid Striped Bass (Morone saxatilis × Morone chrysops)

Seasonal Variations in Fatty Acids of Striped Bass Morone saxatilis

Experimental mycobacteriosis in striped bass Morone saxatilis.

Mycobacterium-Inducible Nramp in Striped Bass (Morone saxatilis)†

Similar Documents

Mycobacterium-inducible Nramp in striped bass (Morone saxatilis).

A unique Mycobacterium species isolated from an epizootic of striped bass (Morone saxatilis).

Genes from the striped bass, morone saxatilis.

Molecular characterization of a rotaviruslike virus isolated from striped bass (Morone saxatilis).

A Unique Mycobacterium Species Isolated from an Epizootic of Striped Bass (Morone saxatilis)

A unique Mycobacterium species isolated from an epizootic of striped bass (Morone saxatilis).

Pasteurellosis in Reared Hybrid Striped Bass (Morone saxatilis × Morone chrysops)

Seasonal Variations in Fatty Acids of Striped Bass Morone saxatilis

Experimental mycobacteriosis in striped bass Morone saxatilis.

Mycobacterium-Inducible Nramp in Striped Bass (Morone saxatilis)†