Experimental mycobacteriosis in striped bass Morone saxatilis.

Content Provider	Semantic Scholar
Author	Gauthier, David T. Rhodes, Martha W. Vogelbein, Wolfgang K. Kator, Howard I. Ottinger, Christopher A.
Copyright Year	2003
Abstract	Striped bass Morone saxatilis were infected intraperitoneally with approximately 10(5) Mycobacterium marinum, M. shottsii sp. nov., or M. gordonae. Infected fish were maintained in a flow-through freshwater system at 18 to 21 degrees C, and were examined histologically and bacteriologically at 2, 4, 6, 8, 17, 26, 36 and 45 wk post-infection (p.i.). M. marinum caused acute peritonitis, followed by extensive granuloma development in the mesenteries, spleen and anterior kidney. Granulomas in these tissues underwent a temporal progression of distinct morphological stages, culminating in well-circumscribed lesions surrounded by normal or healing tissue. Mycobacteria were cultured in high numbers from splenic tissue at all times p.i. Standard Ziehl-Neelsen staining, however, did not demonstrate acid-fast rods in most early inflammatory foci and granulomas. Large numbers of acid-fast rods were present in granulomas beginning at 8 wk p.i. Between 26 and 45 wk p.i., reactivation of disease was observed in some fish, with disintegration of granulomas, renewed inflammation, and elevated splenic bacterial densities approaching 10(9) colony-forming units g(-1). Infection with M. shottsii or M. gordonae did not produce severe pathology. Mild peritonitis was followed by granuloma formation in the mesenteries, but, with 1 exception, granulomas were not observed in the spleen or anterior kidney. M. shottsii and M. gordonae both established persistent infections in the spleen, but were present at densities at least 2 orders of magnitude less than M. marinum at all time points observed. Granulomas in the mesenteries of M. shottsii- and M. gordonae-infected fish resolved over time, and no reactivation of disease was observed.
File Format	PDF HTM / HTML
DOI	10.3354/dao054105
PubMed reference number	12747636
Journal	Medline
Volume Number	54
Issue Number	2
Alternate Webpage(s)	https://digitalcommons.odu.edu/cgi/viewcontent.cgi?article=1188&context=biology_fac_pubs
Alternate Webpage(s)	http://www.int-res.com/articles/dao2003/54/d054p105.pdf
Alternate Webpage(s)	https://scholarworks.wm.edu/cgi/viewcontent.cgi?article=2621&context=vimsarticles
Alternate Webpage(s)	http://digitalcommons.odu.edu/cgi/viewcontent.cgi?article=1188&context=biology_fac_pubs
Alternate Webpage(s)	https://doi.org/10.3354/dao054105
Journal	Diseases of aquatic organisms
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article