NDLI: Purification and Analysis of a Phospholipase A2-Like Lytic Factor of Trichomonas vaginalis

Content Provider	PubMed Central
Author	Lubick, Kirk J. Burgess, Donald E.
Copyright Year	2004
Abstract	Trichomonas vaginalis produces soluble factors that have been reported to have the ability to damage target cells in vitro, and it has been hypothesized that these factors may play a role in the pathogenesis of human trichomoniasis. A lytic factor (LF) was purified from T. vaginalis, and the molecular characteristics of LF were determined. T. vaginalis extract was subjected to hydrophobic chromatography with a 10 to 60% N-propanol gradient in 0.1 M ammonium acetate, resulting in the elution of LF from the column at 30% N-propanol. Cytotoxicity assays revealed that LF was cytotoxic to WEHI 164 cells and bovine red blood cells, and inactivation of LF by treatment with trypsin suggested that the active component of LF was a protein. Size exclusion chromatography of LF produced two fractions at 144 and 168 kDa, and analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of LF under reducing conditions revealed two subunits of 57 and 60 kDa. Results of a fluorescence assay of LF on carboxyfluorescein-labeled liposomes composed of phosphatidylcholine-cholesterol showed that liposomes were hydrolyzed, suggesting that LF had phospholipase activity. Thin-layer chromatography analysis of BODIPY (4,4-difluoro-3a,4adiaza-s-indacene)-labeled phosphatidylcholine treated with LF demonstrated products that migrated identically to the products produced by treatment with phospholipase A2 (PLA2). These results suggest that LF is a PLA2 and may be an important virulence factor of T. vaginalis mediating the destruction of host cells and contributing to tissue damage and inflammation in trichomoniasis.
Related Links	http://dx.doi.org/10.1128/iai.72.3.1284-1290.2004
Ending Page	1290
Page Count	7
Starting Page	1284
File Format	PDF
ISSN	00199567
e-ISSN	10985522
Journal	Infection and Immunity
Issue Number	3
Volume Number	72
Language	English
Publisher	American Society for Microbiology
Publisher Date	2004-03-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Immunology Microbiology Parasitology Infectious Diseases Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Parasitology Immunology Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Natural macrophage cytotoxicity against Trichomonas vaginalis is mediated by soluble lytic factors.

A novel neutrophil-activating factor released by Trichomonas vaginalis.

Trichomonas vaginalis Contact-Dependent Cytolysis of Epithelial Cells

Analysis of the proteinases of representative Trichomonas vaginalis isolates.

Antigen analysis of several pathogenic strains of Trichomonas vaginalis.

Immunogenic and Plasminogen-Binding Surface-Associated α-Enolase of Trichomonas vaginalis ▿

Identification of a surface antigen of Trichomonas vaginalis.

Analysis of human immunoglobulin-degrading cysteine proteinases of Trichomonas vaginalis.

Production of Interleukin-8 by Human Neutrophils Stimulated with Trichomonas vaginalis

Purification and Analysis of a Phospholipase A2-Like Lytic Factor of Trichomonas vaginalis

Similar Documents

Natural macrophage cytotoxicity against Trichomonas vaginalis is mediated by soluble lytic factors.

A novel neutrophil-activating factor released by Trichomonas vaginalis.

Trichomonas vaginalis Contact-Dependent Cytolysis of Epithelial Cells

Analysis of the proteinases of representative Trichomonas vaginalis isolates.

Antigen analysis of several pathogenic strains of Trichomonas vaginalis.

Immunogenic and Plasminogen-Binding Surface-Associated α-Enolase of Trichomonas vaginalis ▿

Identification of a surface antigen of Trichomonas vaginalis.

Analysis of human immunoglobulin-degrading cysteine proteinases of Trichomonas vaginalis.

Production of Interleukin-8 by Human Neutrophils Stimulated with Trichomonas vaginalis

Purification and Analysis of a Phospholipase A2-Like Lytic Factor of Trichomonas vaginalis