NDLI: Cation Dependence, pH Tolerance, and Dosage Requirement of a Bioflocculant Produced by Bacillus spp. UPMB13: Flocculation Performance Optimization through Kaolin Assays

Content Provider	PubMed Central
Author	Zulkeflee, Zufarzaana Aris, Ahmad Zaharin Shamsuddin, Zulkifli H. Yusoff, Md. Kamil
Copyright Year	2012
Abstract	A bioflocculant-producing bacterial strain with highly mucoid and ropy colony morphological characteristics identified as Bacillus spp. UPMB13 was found to be a potential bioflocculant-producing bacterium. The effect of cation dependency, pH tolerance and dosage requirement on flocculating ability of the strain was determined by flocculation assay with kaolin as the suspended particle. The flocculating activity was measured as optical density and by flocs formation. A synergistic effect was observed with the addition of monovalent and divalent cations, namely, Na+, Ca2+, and Mg2+, while Fe2+ and Al3+ produced inhibiting effects on flocculating activity. Divalent cations were conclusively demonstrated as the best cation source to enhance flocculation. The bioflocculant works in a wide pH range, from 4.0 to 8.0 with significantly different performances (P < 0.05), respectively. It best performs at pH 5.0 and pH 6.0 with flocculating performance of above 90%. A much lower or higher pH would inhibit flocculation. Low dosage requirements were needed for both the cation and bioflocculant, with only an input of 50 mL/L for 0.1% (w/v) CaCl2 and 5 mL/L for culture broth, respectively. These results are comparable to other bioflocculants produced by various microorganisms with higher dosage requirements.
Related Links	http://dx.doi.org/10.1100/2012/495659
Starting Page	495659
File Format	PDF
ISSN	1537744X
e-ISSN	1537744X
Journal	The Scientific World Journal
Volume Number	2012
Language	English
Publisher	The Scientific World Journal
Publisher Date	2012-01-01
Access Restriction	Open
Rights Holder	The Scientific World Journal
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Environmental Science(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry, Genetics and Molecular Biology Environmental Science

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