NDLI: Transcriptome and Proteome Research in Veterinary Science: What Is Possible and What Questions Can Be Asked?

Content Provider	PubMed Central
Author	Klopfleisch, Robert Gruber, Achim D.
Copyright Year	2012
Abstract	In recent years several technologies for the complete analysis of the transcriptome and proteome have reached a technological level which allows their routine application as scientific tools. The principle of these methods is the identification and quantification of up to ten thousands of RNA and proteins species in a tissue, in contrast to the sequential analysis of conventional methods such as PCR and Western blotting. Due to their technical progress transcriptome and proteome analyses are becoming increasingly relevant in all fields of biological research. They are mainly used for the explorative identification of disease associated complex gene expression patterns and thereby set the stage for hypothesis-driven studies. This review gives an overview on the methods currently available for transcriptome analysis, that is, microarrays, Ref-Seq, quantitative PCR arrays and discusses their potentials and limitations. Second, the most powerful current approaches to proteome analysis are introduced, that is, 2D-gel electrophoresis, shotgun proteomics, MudPIT and the diverse technological concepts are reviewed. Finally, experimental strategies for biomarker discovery, experimental settings for the identification of prognostic gene sets and explorative versus hypothesis driven approaches for the elucidation of diseases associated genes and molecular pathways are described and their potential for studies in veterinary research is highlighted.
Related Links	http://dx.doi.org/10.1100/2012/254962
Starting Page	254962
File Format	PDF
ISSN	1537744X
e-ISSN	1537744X
Journal	The Scientific World Journal
Volume Number	2012
Language	English
Publisher	The Scientific World Journal
Publisher Date	2012-01-01
Access Restriction	Open
Rights Holder	The Scientific World Journal
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Environmental Science(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry, Genetics and Molecular Biology Environmental Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Understanding endothelial cell apoptosis: what can the transcriptome, glycome and proteome reveal?

The Ipsilesional Upper Limb Can Be Affected following Stroke

Khat Use: What Is the Problem and What Can Be Done?

How Can Stress Be Controlled in Endodontically Treated Teeth? A 3D Finite Element Analysis

Parallel deep transcriptome and proteome analysis of zebrafish larvae

Groundwater protection: What can we learn from Germany?

Arsenic Modulates Posttranslational S-Nitrosylation and Translational Proteome in Keratinocytes

Shadow enhancers: Frequently asked questions about distributed cis-regulatory information and enhancer redundancy

Where There Is No Health Research: What Can Be Done to Fill the Global Gaps in Health Research?

Transcriptome and Proteome Research in Veterinary Science: What Is Possible and What Questions Can Be Asked?

Similar Documents

Understanding endothelial cell apoptosis: what can the transcriptome, glycome and proteome reveal?

The Ipsilesional Upper Limb Can Be Affected following Stroke

Khat Use: What Is the Problem and What Can Be Done?

How Can Stress Be Controlled in Endodontically Treated Teeth? A 3D Finite Element Analysis

Parallel deep transcriptome and proteome analysis of zebrafish larvae

Groundwater protection: What can we learn from Germany?

Arsenic Modulates Posttranslational S-Nitrosylation and Translational Proteome in Keratinocytes

Shadow enhancers: Frequently asked questions about distributed cis-regulatory information and enhancer redundancy

Where There Is No Health Research: What Can Be Done to Fill the Global Gaps in Health Research?

Transcriptome and Proteome Research in Veterinary Science: What Is Possible and What Questions Can Be Asked?