NDLI: Mitigation of H 2 O 2 -Induced Mitochondrial-Mediated Apoptosis in NG108-15 Cells by Novel Mesuagenin C from Mesua kunstleri (King) Kosterm

Content Provider	PubMed Central
Author	Chan, Gomathi Kamarudin, Muhamad Noor Alfarizal Wong, Daniel Zin Hua Ismail, Nor Hadiani Latif, Faizuri Abdul Hasan, Aurengzeb Awang, Khalijah Kadir, Habsah Abdul
Copyright Year	2012
Abstract	This study was aimed to isolate and evaluate neuroprotective compounds from the hexane extract of the bark of Mesua kunstleri (Clusiaceae) on H2O2-induced apoptosis in NG108-15 cells. Five 4-phenylcoumarins were isolated by using various chromatographic techniques via neuroprotective activity-guided fractionation and isolation from the active hexane extract. The chemical structures of the isolated compounds were confirmed by NMR spectroscopic data interpretation and comparison with literature values. Cell viability data demonstrated that mesuagenin C 3 significantly increased cell viability. Hoechst 33342/PI staining illustrated mesuagenin C 3 was able to abate the nuclear shrinkage, chromatin condensation and formation of apoptotic bodies. Pretreatment with mesuagenin C 3 reduced total annexin V positive cells and increased the level of intracellular glutathione (GSH). Mesuagenin C 3 attenuated membrane potential (Δψm), reduced Bax/Bcl-2 ratio and inactivated of caspase-3/7 and -9. These results indicated that mesuagenin C 3 could protect NG108-15 cells against H2O2-induced apoptosis by increasing intracellular GSH level, aggrandizing Δψm, and modulating apoptotic signalling pathway through Bcl-2 family and caspase-3/7 and -9. These findings confirmed the involvement of intrinsic apoptotic pathway in H2O2-induced apoptosis and suggested that mesuagenin C 3 may have potential therapeutic properties for neurodegenerative diseases.
Related Links	http://dx.doi.org/10.1155/2012/156521
Starting Page	156521
File Format	PDF
ISSN	1741427X
e-ISSN	17414288
Journal	Evidence-based Complementary and Alternative Medicine : eCAM
Volume Number	2012
Language	English
Publisher	Hindawi Publishing Corporation
Publisher Date	2012-01-01
Access Restriction	Open
Rights Holder	Hindawi Publishing Corporation
Subject Keyword	Complementary and alternative medicine Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Complementary and Alternative Medicine

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